Cloned (Comment) | Organism |
---|---|
into the vector pQE60 for expression of MurG in Escherichia coli JM109 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E125A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
E268A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
H124A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
H18A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
N127A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
N134A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
N198A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
N291A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
Q288A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
R260A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
S191A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
T15A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
Y105A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | wild-type enzyme | Escherichia coli | |
0.0029 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant R260A | Escherichia coli | |
0.0042 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant N198A | Escherichia coli | |
0.0055 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant T15A | Escherichia coli | |
0.0059 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant Y105A | Escherichia coli | |
0.0071 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant S191A | Escherichia coli | |
0.0085 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant N291A | Escherichia coli | |
0.009 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant H124A | Escherichia coli | |
0.0098 | - |
UDP-GlcNAc | mutant N291A | Escherichia coli | |
0.011 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant E125A | Escherichia coli | |
0.012 | - |
UDP-GlcNAc | mutant E125A | Escherichia coli | |
0.027 | - |
UDP-GlcNAc | wild-type enzyme | Escherichia coli | |
0.033 | - |
UDP-GlcNAc | mutant R260A | Escherichia coli | |
0.038 | - |
UDP-GlcNAc | mutant T15A | Escherichia coli | |
0.047 | - |
UDP-GlcNAc | mutant H124A | Escherichia coli | |
0.05 | - |
UDP-GlcNAc | mutant Y105A | Escherichia coli | |
0.056 | - |
UDP-GlcNAc | mutant N198A | Escherichia coli | |
1.03 | - |
UDP-GlcNAc | mutant S191A | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
determined by SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | Escherichia coli | - |
UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P17443 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni2+-nitrilotriacetate-agarose | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000019 | - |
mutant N127A | Escherichia coli |
0.000063 | - |
mutant E268A | Escherichia coli |
0.00026 | - |
mutant H18A | Escherichia coli |
0.00028 | - |
mutant Q288A | Escherichia coli |
0.00043 | - |
mutant E125A | Escherichia coli |
0.00053 | - |
mutant T15A | Escherichia coli |
0.00067 | - |
mutant H124A | Escherichia coli |
0.0023 | - |
mutant R260A | Escherichia coli |
0.0025 | - |
mutant S191A | Escherichia coli |
0.0064 | - |
mutant N291A | Escherichia coli |
0.0135 | - |
mutant N198A | Escherichia coli |
0.071 | - |
mutant Y105A | Escherichia coli |
0.37 | - |
wild type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli | UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
? | |
UDP-N-acetylglucosamine + MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurG | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
MurG activity assay | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
UDP-GlcNAc | mutant T15A | Escherichia coli | |
0.0016 | - |
UDP-GlcNAc | mutant E125A | Escherichia coli | |
0.0016 | - |
UDP-GlcNAc | mutant H124A | Escherichia coli | |
0.0045 | - |
UDP-GlcNAc | mutant R260A | Escherichia coli | |
0.0055 | - |
UDP-GlcNAc | mutant N291A | Escherichia coli | |
0.038 | - |
UDP-GlcNAc | mutant N198A | Escherichia coli | |
0.093 | - |
UDP-GlcNAc | mutant S191A | Escherichia coli | |
0.123 | - |
UDP-GlcNAc | mutant Y105A | Escherichia coli | |
0.72 | - |
UDP-GlcNAc | wild type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
MurG activity assay | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Escherichia coli |