Literature summary for 2.4.1.227 extracted from

Auger, G.; van Heijenoort, J.; Mengin-Lecreulx, D.; Blanot, D.
A MurG assay which utilises a synthetic analogue of lipid I (2003), FEMS Microbiol. Lett., 219, 115-119.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dimethyl sulfoxide	-	Escherichia coli
Mg2+	-	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
methanol	-	Escherichia coli
trifluoroethanol	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0028	-	MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	-	Escherichia coli
0.15	-	UDP-N-acetylglucosamine	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	-	Escherichia coli	?	-	?
UDP-N-acetylglucosamine + MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydrodecaprenol	very poor substrate	Escherichia coli	UDP + N-acetylglucosamine-MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydrodecaprenol	-	?
UDP-N-acetylglucosamine + MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	-	Escherichia coli	UDP + N-acetylglucosamine-MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	enzyme assay	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Escherichia coli

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Release 2023.1 (January 2023)