Literature summary for 2.4.1.226 extracted from

Izumikawa, T.; Uyama, T.; Okuura, Y.; Sugahara, K.; Kitagawa, H.
Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor (2007), Biochem. J., 403, 545-552.

Search for more literature for 2.4.1.226

Activating Compound

Activating Compound	Comment	Organism	Structure
chondroitin synthase-1	co-expressed with chondroitin synthase-1 CSS3 also exhibits polymerase activity	Homo sapiens
chondroitin-polymerizing factor	co-expressed with ChPF (chondroitin-polymerizing factor) enzyme shows chondroitin polymerase activity	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in COS-1 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D184A	mutant protein which lacks any glycosyltransferase activity but interacts with other ChSy family members shows that the glycosyltransferase activity of CSGlcA-T plays an important role in chondroitin polymerization	Homo sapiens
additional information	overexpression of CSGlcA-T increases the amount of chondroitin sulfate in HeLa cells, whereas the RNA interference of CSGlcA-T results in a reduction of the amount of chondroitin sulphate in the cells	Homo sapiens
additional information	overexpression of CSS3 increases the amount of chondroitin sulfate in HeLa cells, while the RNA interference of CSS3 results in a reduction in the amount of CS in the cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Homo sapiens	5794	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	A2V663	-	-

Purification (Commentary)

Purification (Comment)	Organism
a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source	Homo sapiens

Purification (Comment)

Organism

a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source

Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	CSGlcA-T exhibits polymerization activity on alpha-thrombomodulin using alpha-thrombomodulin as acceptor incubated with UDP-Gal-NAc and UDP-GlcUA. The chain length of chondroitin formed by the co-expressed proteins ChSy-1,ChSy-2 (CSS3), or ChPF in various combinations is different	Homo sapiens	?	-	?
additional information	polymerization reactions carried out using alpha-thrombomodulin as an acceptor co-incubated with UDP-GalNAc and UDP-GlcA	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
chondroitin sulfate glucoronyltransferase	-	Homo sapiens
chondroitin sulfate synthase-3	enzyme possesses dual enzymatic GalNAcT-II (N-acetylgalactosaminyltransferase-II) and GlcAT-II (glucuronyltransferase-II) activities	Homo sapiens
chondroitin synthase-3	-	Homo sapiens
ChSy-3	-	Homo sapiens
CSGlcA-T	enzyme posseses weak GlcAT-II and GalNAcT-II activities	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Homo sapiens