Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of putative catalytic domains of CSGlcAT-II and CSS2 in COS-7 cells as FLAG-tagged soluble proteins, which possesses glucuronyltransferase and N-acetylgalactosaminyltransferase activities as the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
C11-oligosaccharide of chondroitin sulfate A | pH 6.2, 37°C, CSS2 | Homo sapiens | |
0.263 | - |
UDP-alpha-D-glucuronate | pH 6.2, 37°C, CSS2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme is a type II membrane protein | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 17% and 31% as effective as Mn2+ for glucuronyltransferase and N-acetylgalactosaminyltransferase activities, respectively | Homo sapiens | |
Mn2+ | best metal ion, optimal concentration is 10 mM for both activities | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IZ52 | chondroitin sulfate synthase-2, i.e. CSS2; 2 enzymes: CSGlcAT-II and CSS2 | - |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged soluble catalytic domain from COS-7 cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine | enzyme has dual function possessing both glucuronyltransferase and N-acetylgalactosaminyltransferase activities | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | CSS2 is ubiquitously expressed | Homo sapiens | - |
ovary | CSS2, high level expression | Homo sapiens | - |
pancreas | CSS2, high level expression | Homo sapiens | - |
placenta | CSS2, high level expression | Homo sapiens | - |
small intestine | CSS2, high level expression | Homo sapiens | - |
stomach | CSS2, high level expression | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificities of enzymes CSGlcAT-II and CSS2 in truncated form | Homo sapiens | ? | - |
? | |
UDP-alpha-D-glucuronate + C11-oligosaccharide of chondroitin sulfate A | - |
Homo sapiens | ? | - |
? | |
UDP-alpha-D-glucuronate + chondroitin | and oligosaccharide of chondroitin with chain length of C11 | Homo sapiens | UDP + beta-D-glucuronosyl-(1,3)-chondroitin | - |
? | |
UDP-alpha-D-glucuronate + chondroitin sulfate A | and oligosaccharide of chondroitin sulfate with chain length of C11 | Homo sapiens | UDP + beta-D-glucuronosyl-(1,3)-chondroitin sulfate A | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modeling of the C-terminus | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
chondroitin sulfate glucuronyltransferase | - |
Homo sapiens |
chondroitin sulfate synthase-2 | - |
Homo sapiens |
CSGlcAT-II | - |
Homo sapiens |
CSS2 | - |
Homo sapiens |
GlcAT-II | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
assay at | Homo sapiens |