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Literature summary for 2.4.1.222 extracted from

  • Peterson, R.E.; McClay, D.R.
    A Fringe-modified Notch signal affects specification of mesoderm and endoderm in the sea urchin embryo (2005), Dev. Biol., 282, 126-137.
    View publication on PubMed
Search for more literature for 2.4.1.222

Cloned(Commentary)

Cloned (Comment) Organism
DNA acid sequence determination and analysis, expression as insoluble His-tagged protein in Escherichia coli strain XL1-Blue Lytechinus variegatus

Protein Variants

Protein Variants Comment Organism
additional information enzyme expression is perturbed by injection of fringe morpholino antisense oligonucleotide resulting in a reduced number of secondary mesenchym cells and altered endoderm and mesoderm specification, as well as decreased and delayed archenteron invagination Lytechinus variegatus

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme expression is perturbed by injection of fringe morpholino antisense oligonucleotide resulting in a reduced number of secondary mesenchym cells and altered endoderm and mesoderm specification, as well as decreased and delayed archenteron invagination Lytechinus variegatus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Lytechinus variegatus 5794
-
membrane apical and surface membranes Lytechinus variegatus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Notch + UDP-D-GlcNAc Lytechinus variegatus glycosylation of Notch enables it to activate signaling through binding to Delta ligand required for the specification of secondary mesenchym cells, loss of enzyme delays ectoderm patterning beta-D-glucosaminyl-Notch + UDP
-
 ?

Organism

Organism UniProt Comment Textmining
Lytechinus variegatus
-
 sea urchin
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity chromatography Lytechinus variegatus

Source Tissue

Source Tissue Comment Organism Textmining
archenteron invaginated Lytechinus variegatus
-
embryo expression is relatively constant during germ layer development and occurs in all cells, but becomes restricted during gastrulation Lytechinus variegatus
-
endoderm
-
 Lytechinus variegatus
-
additional information distribution, Fringe is expressed in both the signaling and the receiving cells during the first Delta-Notch signal Lytechinus variegatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Notch + UDP-D-GlcNAc glycosylation of Notch enables it to activate signaling through binding to Delta ligand required for the specification of secondary mesenchym cells, loss of enzyme delays ectoderm patterning Lytechinus variegatus beta-D-glucosaminyl-Notch + UDP
-
 ?
Notch + UDP-D-GlcNAc Notch protein is O-fucosylated at serine or threonine residues in the extracellular epidermal growth factor-like repeats, enzyme is O-fucose-specific Lytechinus variegatus beta-D-glucosaminyl-Notch + UDP
-
 ?

Synonyms

Synonyms Comment Organism
beta1,3-N-acetylglucosaminyltransferase
-
 Lytechinus variegatus