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Literature summary for 2.4.1.22 extracted from
- A pH-sensitive assay for galactosyltransferase (2004), Anal. Biochem., 330, 219-226.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Neisseria meningitidis |
| expression in Escherichia coli | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-lactalbumin | inhibits galactosyl transfer to N-acetylglucosamine | Helicobacter pylori |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required, optimal metal cofactor at 0.1 mM MnCl2 | Neisseria meningitidis |  |
| Mn2+ | required, optimal metal cofactor at 0.1 mM MnCl2 | Helicobacter pylori | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
| Neisseria meningitidis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme fro Escherichia coli | Neisseria meningitidis |
| recombinant enzyme fro Escherichia coli | Helicobacter pylori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of a simple and fast pH-sensitive assay method using phenol red as pH-indicator, activities at different temperatures, overview | Helicobacter pylori |
| additional information | - |
development of a simple and fast pH-sensitive assay method using phenol red as pH-indicator, overview | Neisseria meningitidis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | acceptor substrate specificity, no activity with D-galactose, N-acetylgalactosamine, glucosamine, and lactose as acceptor substrates, no activity with UDP-glucose as donor substrate, overview | Helicobacter pylori | ? | - |
? | |
| additional information | acceptor substrate specificity, no activity with D-galactose, N-acetylgalactosamine, glucosamine, and lactose as acceptor substrates, overview | Neisseria meningitidis | ? | - |
? | |
| UDP-galactose + D-glucose | - |
Neisseria meningitidis | UDP + lactose | - |
? | |
| UDP-galactose + D-glucose | - |
Helicobacter pylori | UDP + lactose | - |
? | |
| UDP-galactose + N-acetyl-D-glucosamine | - |
Neisseria meningitidis | UDP + D-galactosyl(1-4)beta-N-acetyl-D-glucosamine | - |
? | |
| UDP-galactose + N-acetyl-D-glucosamine | - |
Helicobacter pylori | UDP + D-galactosyl(1-4)beta-N-acetyl-D-glucosamine | - |
? | |
| UDP-glucose + N-acetyl-D-glucosamine | about 20% activity compared to UDP-galactose | Neisseria meningitidis | UDP + D-glucosyl(1-4)beta-N-acetyl-D-glucosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta1,4-galactosyltransferase | - |
Neisseria meningitidis |
| beta1,4-galactosyltransferase | - |
Helicobacter pylori |
| Gal-T1 | - |
Neisseria meningitidis |
| Gal-T1 | - |
Helicobacter pylori |
| HP0826 | - |
Helicobacter pylori |
| LgtB | - |
Neisseria meningitidis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Neisseria meningitidis |
| 30 | - |
- |
Helicobacter pylori |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 37 | - |
Neisseria meningitidis |
| 20 | 37 | - |
Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
about | Neisseria meningitidis |
| 8 | - |
about | Helicobacter pylori |
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Release 2023.1 (January 2023)
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