Literature summary for 2.4.1.218 extracted from

Shen, X.; Wang, J.; Wang, J.; Chen, Z.; Yuan, Q.; Yan, Y.
High-level de novo biosynthesis of arbutin in engineered Escherichia coli (2017), Metab. Eng., 42, 52-58 .

Search for more literature for 2.4.1.218

Application

Application	Comment	Organism
synthesis	an artificial pathway is established in Escherichia coli for high-level production of arbutin from simple carbon sources in Escherichia coli for high-level production of arbutin from simple carbon sources. Introduction of the genes for 4-hydroxybenzoate 1-hydroxylase from Candida parapsilosis CBS604 and hydroquinone glucosyltransferase from Rauvolfia serpentina into Escherichia coli leads to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments	Rauvolfia serpentina

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	hydroquinone	pH 7.5, 30°C	Rauvolfia serpentina

Organism

Organism	UniProt	Comment	Textmining
Rauvolfia serpentina	Q9AR73	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + hydroquinone	-	Rauvolfia serpentina	UDP + hydroquinone-O-beta-D-glucopyranoside	-	?

Synonyms

Synonyms	Comment	Organism
arbutin synthase	-	Rauvolfia serpentina

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00038	-	hydroquinone	pH 7.5, 30°C	Rauvolfia serpentina

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0009	-	hydroquinone	pH 7.5, 30°C	Rauvolfia serpentina

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Release 2023.1 (January 2023)