Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.218 extracted from

  • Hefner, T.; Arend, J.; Warzecha, H.; Siems, K.; Stöckigt, J.
    Arbutin synthase, a novel member of the NRD1beta glycosyltransferase family, is a unique multifunctional enzyme converting various natural products and xenobiotics (2002), Bioorg. Med. Chem., 10, 1731-1741.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA with 6xHis tag Rauvolfia serpentina
expression in Escherichia coli Rauvolfia serpentina

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, 24.4% inhibition Rauvolfia serpentina
Co2+ 10 mM, 54.2% inhibition Rauvolfia serpentina
dichlorophen
-
Rauvolfia serpentina
Hg2+ 10 mM, complete inhibition, reversed by addition of 20 mM 2-mercaptoethanol Rauvolfia serpentina
Mn2+ 10 mM, 24.7% inhibition Rauvolfia serpentina
Zn2+ 10 mM, 87.4% inhibition Rauvolfia serpentina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value for hydroquinone is below 0.001 mM Rauvolfia serpentina
0.077
-
UDPglucose
-
Rauvolfia serpentina
0.44
-
Vanillin
-
Rauvolfia serpentina
0.55
-
umbelliferone
-
Rauvolfia serpentina
1.1
-
TDPglucose
-
Rauvolfia serpentina

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, increases the conversion rate to 168.3% Rauvolfia serpentina

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina Q9AR73
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rauvolfia serpentina

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Rauvolfia serpentina
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-glucose + hydroquinone 2% of the activity with UDPglucose Rauvolfia serpentina CDP + hydroquinone-O-beta-D-glucopyranoside
-
?
additional information no activity with UDPmannose, UDPgalactose or UDPglucuronic acid Rauvolfia serpentina ?
-
?
TDP-glucose + hydroquinone 68.8% of the activity with UDPglucose Rauvolfia serpentina TDP + hydroquinone-O-beta-D-glucopyranoside
-
?
UDP-alpha-D-glucose + 3-hydroxyflavone 5.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 3-[(beta-D-glucopyranosyl)oxy]-flavone
-
?
UDP-alpha-D-glucose + biphenyl-2-ol 1.1% of the activity with hydroquinone Rauvolfia serpentina UDP + [1,1'-biphenyl]-2-yl beta-D-glucopyranoside
-
?
UDP-alpha-D-glucose + kaempferol 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + ?
-
?
UDP-alpha-D-glucose + quercetin 2.6% of the activity with hydroquinone Rauvolfia serpentina UDP + ?
-
?
UDP-glucose + 2-methoxy-hydroquinone 16.7% of the activity with hydroquinone Rauvolfia serpentina UDP + 1-O-(4-hydroxy-2-methoxyphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 3-methoxyphenol 10.9% of the activity with hydroquinone Rauvolfia serpentina UDP + 1-O-(3-methoxyphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-benzyloxyphenol 1.4% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-benzyloxyphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-chloro-2-methylphenol 19.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 1-O-(4-chloro-2-methylphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-chlorophenol 7.5% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-chlorophenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-hydroxyacetophenone ,1.8% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-acetophenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-hydroxybenzaldehyde 8.6% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-formylphenyl beta-D-glucopyranoside
-
?
UDP-glucose + 4-hydroxythiophenol 1.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-thiophenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-methylphenol 2.8% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-methylphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 4-nitrophenol 6.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-nitrophenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + 7-hydroxyflavone 3.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 7-beta-D-glucopyranosyloxy-2-phenylchromen-4-one
-
?
UDP-glucose + apigenin 5.8% of the activity with hydroquinone Rauvolfia serpentina UDP + ?
-
?
UDP-glucose + carvacrol 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + (2-methyl-5-isopropylphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + coniferyl alcohol 1.2% of the activity with hydroquinone Rauvolfia serpentina UDP + coniferin
-
?
UDP-glucose + eugenol 6.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-allyl-2-methoxyphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + hydroquinone
-
Rauvolfia serpentina UDP + hydroquinone-O-beta-D-glucopyranoside
-
?
UDP-glucose + methylvanillate 2.2% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid
-
?
UDP-glucose + phenol 5.1% of the activity with hydroquinone Rauvolfia serpentina UDP + phenyl-beta-D-glucopyranoside
-
?
UDP-glucose + resorcinol 8.7% of the activity with hydroquinone Rauvolfia serpentina UDP + (3-hydroxyphenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + saligenin 9.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 2-(hydroxymethyl)phenyl beta-D-glucopyranoside
-
?
UDP-glucose + scopoletin 3.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 7-beta-D-glucopyranosyloxy-6-methoxychromen-2-one
-
?
UDP-glucose + thymol 7.6% of the activity with hydroquinone Rauvolfia serpentina UDP + (2-isopropyl-5-methyl-phenyl)-beta-D-glucopyranoside
-
?
UDP-glucose + umbelliferone 3.6% of the activity with hydroquinone Rauvolfia serpentina UDP + 7-beta-D-glucopyranosyloxy-chromen-2-one
-
?
UDP-glucose + vanillic acid 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid
-
?
UDP-glucose + vanillin 9.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-beta-D-glucopyranosyloxy-3-methoxy-benzaldehyde
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant enzyme Rauvolfia serpentina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
and a second optimum at pH 6.8 in phosphate buffer, and a second optimum at pH 7.5 in citric acid buffer Rauvolfia serpentina
6.8
-
and a second optimum at pH 4.5, phosphate buffer Rauvolfia serpentina
7.5
-
and a second optimum at pH 4.5, citric acid buffer Rauvolfia serpentina