Cloned (Comment) | Organism |
---|---|
cDNA with 6xHis tag | Rauvolfia serpentina |
expression in Escherichia coli | Rauvolfia serpentina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 24.4% inhibition | Rauvolfia serpentina | |
Co2+ | 10 mM, 54.2% inhibition | Rauvolfia serpentina | |
dichlorophen | - |
Rauvolfia serpentina | |
Hg2+ | 10 mM, complete inhibition, reversed by addition of 20 mM 2-mercaptoethanol | Rauvolfia serpentina | |
Mn2+ | 10 mM, 24.7% inhibition | Rauvolfia serpentina | |
Zn2+ | 10 mM, 87.4% inhibition | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for hydroquinone is below 0.001 mM | Rauvolfia serpentina | |
0.077 | - |
UDPglucose | - |
Rauvolfia serpentina | |
0.44 | - |
Vanillin | - |
Rauvolfia serpentina | |
0.55 | - |
umbelliferone | - |
Rauvolfia serpentina | |
1.1 | - |
TDPglucose | - |
Rauvolfia serpentina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM, increases the conversion rate to 168.3% | Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | Q9AR73 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rauvolfia serpentina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rauvolfia serpentina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-glucose + hydroquinone | 2% of the activity with UDPglucose | Rauvolfia serpentina | CDP + hydroquinone-O-beta-D-glucopyranoside | - |
? | |
additional information | no activity with UDPmannose, UDPgalactose or UDPglucuronic acid | Rauvolfia serpentina | ? | - |
? | |
TDP-glucose + hydroquinone | 68.8% of the activity with UDPglucose | Rauvolfia serpentina | TDP + hydroquinone-O-beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + 3-hydroxyflavone | 5.4% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 3-[(beta-D-glucopyranosyl)oxy]-flavone | - |
? | |
UDP-alpha-D-glucose + biphenyl-2-ol | 1.1% of the activity with hydroquinone | Rauvolfia serpentina | UDP + [1,1'-biphenyl]-2-yl beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + kaempferol | 1.0% of the activity with hydroquinone | Rauvolfia serpentina | UDP + ? | - |
? | |
UDP-alpha-D-glucose + quercetin | 2.6% of the activity with hydroquinone | Rauvolfia serpentina | UDP + ? | - |
? | |
UDP-glucose + 2-methoxy-hydroquinone | 16.7% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 1-O-(4-hydroxy-2-methoxyphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 3-methoxyphenol | 10.9% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 1-O-(3-methoxyphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-benzyloxyphenol | 1.4% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-benzyloxyphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-chloro-2-methylphenol | 19.0% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 1-O-(4-chloro-2-methylphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-chlorophenol | 7.5% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-chlorophenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-hydroxyacetophenone | ,1.8% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-acetophenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-hydroxybenzaldehyde | 8.6% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 4-formylphenyl beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-hydroxythiophenol | 1.2% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-thiophenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-methylphenol | 2.8% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-methylphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-nitrophenol | 6.2% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-nitrophenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + 7-hydroxyflavone | 3.0% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 7-beta-D-glucopyranosyloxy-2-phenylchromen-4-one | - |
? | |
UDP-glucose + apigenin | 5.8% of the activity with hydroquinone | Rauvolfia serpentina | UDP + ? | - |
? | |
UDP-glucose + carvacrol | 1.0% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (2-methyl-5-isopropylphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + coniferyl alcohol | 1.2% of the activity with hydroquinone | Rauvolfia serpentina | UDP + coniferin | - |
? | |
UDP-glucose + eugenol | 6.2% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (4-allyl-2-methoxyphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + hydroquinone | - |
Rauvolfia serpentina | UDP + hydroquinone-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + methylvanillate | 2.2% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid | - |
? | |
UDP-glucose + phenol | 5.1% of the activity with hydroquinone | Rauvolfia serpentina | UDP + phenyl-beta-D-glucopyranoside | - |
? | |
UDP-glucose + resorcinol | 8.7% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (3-hydroxyphenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + saligenin | 9.4% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 2-(hydroxymethyl)phenyl beta-D-glucopyranoside | - |
? | |
UDP-glucose + scopoletin | 3.0% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 7-beta-D-glucopyranosyloxy-6-methoxychromen-2-one | - |
? | |
UDP-glucose + thymol | 7.6% of the activity with hydroquinone | Rauvolfia serpentina | UDP + (2-isopropyl-5-methyl-phenyl)-beta-D-glucopyranoside | - |
? | |
UDP-glucose + umbelliferone | 3.6% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 7-beta-D-glucopyranosyloxy-chromen-2-one | - |
? | |
UDP-glucose + vanillic acid | 1.0% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid | - |
? | |
UDP-glucose + vanillin | 9.4% of the activity with hydroquinone | Rauvolfia serpentina | UDP + 4-beta-D-glucopyranosyloxy-3-methoxy-benzaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Rauvolfia serpentina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
and a second optimum at pH 6.8 in phosphate buffer, and a second optimum at pH 7.5 in citric acid buffer | Rauvolfia serpentina |
6.8 | - |
and a second optimum at pH 4.5, phosphate buffer | Rauvolfia serpentina |
7.5 | - |
and a second optimum at pH 4.5, citric acid buffer | Rauvolfia serpentina |