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Literature summary for 2.4.1.217 extracted from

  • Empadinhas, N.; Albuquerque, L.; Henne, A.; Santos, H.; Da Costa, M.
    The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate (2003), Appl. Environ. Microbiol., 69, 3272-3279 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.217

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
KCl 50 to 300 mM Thermus thermophilus
Mn2+ 20 mM Thermus thermophilus
NaCl 50 to 300 mM Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 3-phospho-D-glycerate pH 7.6, 70°C Thermus thermophilus
0.33
-
 GDP-alpha-D-mannose pH 7.6, 70°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity is strictly dependent on divalent cations, Mn2+ is most effective (between 0.2 and 2.0 mM), Mg2+x01has a similar effect on the activation at about 20.0 mM Thermus thermophilus
Mn2+ activity is strictly dependent on divalent cations, Mn2+ is most effective, maximum activation of the enzyme by Mn2+ is between 0.2 and 2.0 mM Thermus thermophilus
additional information Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate activity at any concentration Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43600
-
 calculated from sequence Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q84B24
-
-
Thermus thermophilus DSM 7039 Q84B24
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
GDP-alpha-D-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus DSM 7039 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Synonyms

Synonyms Comment Organism
MPGS
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 103 25°C: 10% of the maximal activity, 103°C: 55% of maximal activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life: 189 min Thermus thermophilus
90
-
 half-life: 22 min Thermus thermophilus
100
-
 half-life: 10 min Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermus thermophilus