Literature summary for 2.4.1.217 extracted from

Goncalves, S.; Borges, N.; Esteves, A.M.; Victor, B.L.; Soares, C.M.; Santos, H.; Matias, P.M.
Structural analysis of Thermus thermophilus HB27 mannosyl-3-phosphoglycerate synthase provides evidence for a second catalytic metal ion and new insight into the retaining mechanism of glycosyltransferases (2010), J. Biol. Chem., 285, 17857-17868.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermus thermophilus HB27

Crystallization (Commentary)

Crystallization (Comment)	Organism
the three-dimensional structure of mannosyl-3-phosphoglycerate synthase in its binary complex form, with GDP-alpha-D-mannose and Mg2+, shows a second metal binding site, about 6 A away from the mannose moiety, wild-type and H309A mutant	Thermus thermophilus HB27

Protein Variants

Protein Variants	Comment	Organism
E251A	site-directed mutagenesis, no specific activity detected	Thermus thermophilus HB27
H309A	site-directed mutagenesis with the absence of the metal site, negligible specific activity	Thermus thermophilus HB27

Inhibitors

Inhibitors	Comment	Organism	Structure
Zn2+	measurement in the presence of Zn2+ (300 microM) instead Mg2+, the activity decreases about 100fold (0.4 micromol/min/mg), Zn2+ can displace Mg2+ at the second metal binding site	Thermus thermophilus HB27

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	used as single metal cofactor at 20 mM, activity dependend on	Thermus thermophilus HB27

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus HB27	Q84B24	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus HB27

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.4	-	with Zn2+ as a cofactor	Thermus thermophilus HB27
5.3	-	when 20 mM Mg2+ is added in combination with Zn2+ (300 microM), the MpgS specific activity recovered substantially	Thermus thermophilus HB27
45	-	with Mg2+ as a cofactor	Thermus thermophilus HB27

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-mannose + 3-phospho-D-glycerate	-	Thermus thermophilus HB27	GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Thermus thermophilus HB27

Synonyms

Synonyms	Comment	Organism
mannosyl-3-phosphoglycerate synthase	-	Thermus thermophilus HB27
MPGS	glycosyltransferase from the GT55 family	Thermus thermophilus HB27

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	assay at	Thermus thermophilus HB27

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Thermus thermophilus HB27

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Release 2023.1 (January 2023)