Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Caenorhabditis elegans |
expressed in Pichia pastoris strain GS115 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D303E | the mutant retains 23.8% of the wild type activity | Caenorhabditis elegans |
N194Q | glycosylation site mutant with less than 1% of wild type activity | Caenorhabditis elegans |
N219A | the mutant retains 1.2% of the wild type activity | Arabidopsis thaliana |
N337A | the mutant retains 18.6% of the wild type activity | Arabidopsis thaliana |
R226A | the mutation leads to a complete loss of activity | Arabidopsis thaliana |
S218A | the mutation completely abolishes the enzyme activity | Arabidopsis thaliana |
S243A | the mutant retains 16.2% of the wild type activity | Caenorhabditis elegans |
S253A | the mutant retains 35.6% of the wild type activity | Arabidopsis thaliana |
S483A | the mutant retains 32.2% of the wild type activity | Arabidopsis thaliana |
T339A | the mutant retains 12.1% of the wild type activity | Arabidopsis thaliana |
T361A | the mutant retains 24.3% of the wild type activity | Caenorhabditis elegans |
T422A | the mutant retains 36.5% of the wild type activity | Arabidopsis thaliana |
Y243A | the mutant retains 9.8% of the wild type activity | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 3% residual activity in the presence of 1 mM | Arabidopsis thaliana | |
Cu2+ | 3% residual activity in the presence of 1 mM | Caenorhabditis elegans | |
EDTA | about 5% residual activity in the presence of 10 mM | Arabidopsis thaliana | |
EDTA | about 5% residual activity in the presence of 10 mM | Caenorhabditis elegans | |
Zn2+ | 3% residual activity in the presence of 1 mM | Arabidopsis thaliana | |
Zn2+ | 3% residual activity in the presence of 1 mM | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
GDP-beta-L-fucose | mutant enzyme S253A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C | Arabidopsis thaliana | |
0.24 | - |
GDP-beta-L-fucose | mutant enzyme Y243A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C | Arabidopsis thaliana | |
0.33 | - |
GDP-beta-L-fucose | mutant enzyme N219A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C | Arabidopsis thaliana | |
0.36 | - |
GDP-beta-L-fucose | mutant enzyme T339A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C | Arabidopsis thaliana | |
1 | - |
GDP-beta-L-fucose | wild type enzyme, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Caenorhabditis elegans | |
Ca2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Arabidopsis thaliana | |
Co2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Caenorhabditis elegans | |
Co2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Arabidopsis thaliana | |
Mg2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Caenorhabditis elegans | |
Mg2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Arabidopsis thaliana | |
Mn2+ | highest relative enzyme activity in the presence of Mn2+ | Caenorhabditis elegans | |
Mn2+ | highest relative enzyme activity in the presence of Mn2+ | Arabidopsis thaliana | |
additional information | Zn2+ and Cu2+ ions do not activate the enzyme | Caenorhabditis elegans | |
additional information | Zn2+ and Cu2+ ions do not activate the enzyme | Arabidopsis thaliana | |
Ni2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Caenorhabditis elegans | |
Ni2+ | the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, calculated from amino acid sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1-2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc | Arabidopsis thaliana | - |
GDP + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(Fucalpha(1->3))GlcNAc | - |
? | |
GDP-beta-L-fucose + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc | Caenorhabditis elegans | - |
GDP + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C8W3 | - |
- |
Caenorhabditis elegans | G5EDR5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Caenorhabditis elegans |
glycoprotein | - |
Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
Ni-NTA or HisTrap column chromatography | Caenorhabditis elegans |
Ni-NTA or HisTrap column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1-2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc | - |
Arabidopsis thaliana | GDP + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(Fucalpha(1->3))GlcNAc | - |
? | |
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc | - |
Arabidopsis thaliana | GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc | - |
? | |
GDP-beta-L-fucose + Manalpha(1->6)(Manalpha(1->3))Manalpha(1->6)(Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc | - |
Caenorhabditis elegans | GDP + Manalpha(1->6)(Manalpha(1->3))Manalpha(1->6)(Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)[Fucalpha(1->3)]GlcNAc | - |
? | |
GDP-beta-L-fucose + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc | - |
Caenorhabditis elegans | GDP + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, calculated from amino acid sequence | Arabidopsis thaliana |
? | x * 60000-70000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
core alpha1,3-fucosyltransferase | - |
Caenorhabditis elegans |
core alpha1,3-fucosyltransferase | - |
Arabidopsis thaliana |
FucTA | - |
Arabidopsis thaliana |
FUT-1 | - |
Caenorhabditis elegans |
FUT11 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Caenorhabditis elegans |
30 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Arabidopsis thaliana |
7.5 | - |
- |
Caenorhabditis elegans |