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Literature summary for 2.4.1.214 extracted from

  • Rendic, D.; Klaudiny, J.; Stemmer, U.; Schmidt, J.; Paschinger, K.; Wilson, I.B.
    Towards abolition of immunogenic structures in insect cells: characterization of a honey-bee (Apis mellifera) multi-gene family reveals both an allergy-related core alpha1,3-fucosyltransferase and the first insect Lewis-histo-blood-group-related antigen-synthesizing enzyme (2007), Biochem. J., 402, 603.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris GS115 cells Apis mellifera
expression in Sf9 cells Apis mellifera
the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity Apis mellifera

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ completely abolishes the enzyme’s activity Apis mellifera
EDTA completely abolishes the enzyme’s activity Apis mellifera
Zn2+ completely abolishes the enzyme’s activity Apis mellifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ strongly activated by Mn2+ Apis mellifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 calculation from sequence of cDNA Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera Q05GU3 honey-bee
-

Source Tissue

Source Tissue Comment Organism Textmining
brain predominantly expressed Apis mellifera
-
hypopharynx
-
 Apis mellifera
-
larva
-
 Apis mellifera
-
thorax muscle
-
 Apis mellifera
-
venom gland predominantly expressed Apis mellifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn
-
 Apis mellifera GDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)[Fucalpha1-6]GlcNAcbeta1-Asn difucosylated glycan structure ?
GDP-fucose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn
-
 Apis mellifera GDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn
-
 ?
additional information substrate specificity of FucTA of Apis mellifera is similar to Drosophila melanogaster FucTA Apis mellifera ?
-
 ?

Synonyms

Synonyms Comment Organism
core alpha1,3-fucosyltransferase
-
 Apis mellifera
FucTA
-
 Apis mellifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
-
 Apis mellifera

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 enzyme shows maximal activity in mildly alkaline conditions Apis mellifera