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Literature summary for 2.4.1.214 extracted from
- Production of soluble human alpha3-fucosyltransferase (FucT VII) by membrane targeting and in vivo proteolysis (2001), Glycobiology, 11, 711-717.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the enzyme is a target for rational inhibitor design for medication in the treatment of rheumatoid arthritis | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the soluble enzyme, by constructing a fusion enzyme amino acid residues 1-63 of a mammalian enzyme fused to the stem and the catalytic region of residues 39-342 of the enzyme, in CHO cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | slight inhibition at 3-5 mM | Homo sapiens |  |
| CDP | slight inhibition at 3-5 mM | Homo sapiens | |
| Cu2+ | - |
Homo sapiens | |
| Fe2+ | - |
Homo sapiens | |
| GDP | strong product inhibition, complete inhibition at 5 mM | Homo sapiens | |
| GMP | strong inhibition | Homo sapiens | |
| GTP | strong inhibition | Homo sapiens | |
| additional information | no inhibition of the recombinant soluble enzyme by NEM | Homo sapiens | |
| NaCl | 50% inhibition at 250 mM, complete inhibition at 500 mM and above | Homo sapiens | |
| UDP | slight inhibition at 3-5 mM | Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0062 | - |
GDP-alpha-L-fucose | recombinant soluble enzyme | Homo sapiens | |
| 0.5 | - |
fetuin | recombinant soluble enzyme | Homo sapiens | |
| 5.6 | - |
NeuAcalpha2-3Galbeta1-4GlcNAc | recombinant soluble enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | lumenal side of the membrane | Homo sapiens | 5794 | - |
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | less effective than Mn2+ | Homo sapiens | |
| Co2+ | less effective than Mn2+ | Homo sapiens | |
| Mg2+ | less effective than Mn2+ | Homo sapiens | |
| Mn2+ | activates best at 15 mM | Homo sapiens | |
| additional information | activation by divalent cations in descending order: Mn2+, Mg2+, Ca2+, Co2+, while Ni2+ has no effect on the enzyme activity | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 49000, recombinant enzyme glycoform 1, SDS-PAGE, x * 55000, recombinant enzyme glycoform 1, SDS-PAGE | Homo sapiens |
| 55000 | - |
x * 49000, recombinant enzyme glycoform 1, SDS-PAGE, x * 55000, recombinant enzyme glycoform 1, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-L-fucose + Galbeta1-4GlcNAc-R | Homo sapiens | - |
GDP + Galbeta1-4[Fucalpha1-3]GlcNAc-R | - |
ir | |
| additional information | Homo sapiens | the enzyme synthesizes functional selectin ligands on the surface of leukocytes | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant enzyme exists in 2 glycoprotein forms of 55 and 49 kDa, cleaving by PNGaseF | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble enzyme from CHO cells by GDP-hexanolamine affinity chromatography to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.6 | - |
purified recombinant soluble enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-L-fucose + fetuin | - |
Homo sapiens | GDP + 3-alpha-L-fucosyl-fetuin | - |
? | |
| GDP-alpha-L-fucose + Galbeta1-4GlcNAc-R | - |
Homo sapiens | GDP + Galbeta1-4[Fucalpha1-3]GlcNAc-R | - |
ir | |
| GDP-alpha-L-fucose + NeuAcalpha2-3Galbeta1-3GlcNAcbeta-R | 4% of the activity with NeuAcalpha2-3Galbeta1-4GlcNAc-R | Homo sapiens | GDP + NeuAcalpha2-3Galbeta1-3[Fucalpha1-3]GlcNAcbeta-R | - |
? | |
| GDP-alpha-L-fucose + NeuAcalpha2-3Galbeta1-4GlcNAc | 28% of the activity with NeuAcalpha2-3Galbeta1-4GlcNAc-R | Homo sapiens | GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc | - |
? | |
| GDP-alpha-L-fucose + NeuAcalpha2-3Galbeta1-4GlcNAcbeta-R | best substrate | Homo sapiens | GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAcbeta-R | - |
? | |
| additional information | the enzyme synthesizes functional selectin ligands on the surface of leukocytes | Homo sapiens | ? | - |
? | |
| additional information | poorly active with Galbeta1-4GlcNAc-O-(CH2)8-COOCH3, Fucalpha1-2Galbeta1-4GlcNAcbeta-R, Galbeta1-3GlcNAcbeta-R, and Fucalpha1-2Galbeta1-3GlcNAcbeta-R | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, recombinant enzyme glycoform 1, SDS-PAGE, x * 55000, recombinant enzyme glycoform 1, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha3-fucosyltransferase | - |
Homo sapiens |
| FucT VII | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | the recombinant enzyme shows a broad pH-optimum | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | enzyme is not affected by high pH but shows no activity at pH below pH 5.5 | Homo sapiens |
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