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Literature summary for 2.4.1.214 extracted from

  • Staudacher, E.; Dalik, T.; Wawra, P.; Altmann, F.; Maerz, L.
    Functional purification and characterization of a GDP-fucose: beta-N-acetylglucosamine (Fuc to Asn linked GlcNAc) alpha1,3-fucosyltransferase from mung beans (1995), Glycoconj. J., 12, 780-786.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.1-0.5% slightly enhances activity Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide not inhibitory Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
GnGnF6-peptide
-
Vigna radiata
0.26
-
GnGn-peptide
-
Vigna radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ optimal activity at 10-15 mM Vigna radiata
Zn2+
-
Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration Vigna radiata
65000
-
1 * 65000, SDS-PAGE Vigna radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by chromatography on DE52 cellulose and Affigel Blue, chromatofocusing using PBE 94 and polybuffer 96, gelfiltration on a Sephacryl S200 column and affinity chromatography on a GnGn-column Vigna radiata

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Vigna radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.071
-
-
Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + GnGn-oligosaccharide 93% relative activity of pure enzyme Vigna radiata GDP + alpha1-3 fucosylated GnGn-oligosaccharide
-
?
GDP-fucose + GnGn-peptide 100% relative activity of pure enzyme and of crude extract Vigna radiata GDP + alpha1-3 fucosylated peptide
-
?
GDP-fucose + GnGnF6-oligosaccharide 95% relative activity of pure enzyme Vigna radiata GDP + alpha1-3 fucosylated oligosaccharide
-
?
GDP-fucose + GnGnF6-peptide 99% relative activity of pure enzyme and 70% relative activity of crude extract Vigna radiata GDP + alpha1-3-fucosylated GnGnF6-peptide
-
?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
Vigna radiata GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
?
additional information the enzyme acts upon N-glycopeptides and related oligosaccharides with the glycan structure GlcNAc2Man3GlcNAc2, no transfer to N-glycans is observed when the terminal GlcNAc residues are either absent or substituted with galactose, N-acetyllactosamine, lacto-N-biose and N-acetylchito-oligosaccharides do not function as acceptors for the enzyme Vigna radiata ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Vigna radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Vigna radiata