Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.1-0.5% slightly enhances activity | Vigna radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | not inhibitory | Vigna radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
GnGnF6-peptide | - |
Vigna radiata | |
0.26 | - |
GnGn-peptide | - |
Vigna radiata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | optimal activity at 10-15 mM | Vigna radiata | |
Zn2+ | - |
Vigna radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
gel filtration | Vigna radiata |
65000 | - |
1 * 65000, SDS-PAGE | Vigna radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by chromatography on DE52 cellulose and Affigel Blue, chromatofocusing using PBE 94 and polybuffer 96, gelfiltration on a Sephacryl S200 column and affinity chromatography on a GnGn-column | Vigna radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Vigna radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.071 | - |
- |
Vigna radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + GnGn-oligosaccharide | 93% relative activity of pure enzyme | Vigna radiata | GDP + alpha1-3 fucosylated GnGn-oligosaccharide | - |
? | |
GDP-fucose + GnGn-peptide | 100% relative activity of pure enzyme and of crude extract | Vigna radiata | GDP + alpha1-3 fucosylated peptide | - |
? | |
GDP-fucose + GnGnF6-oligosaccharide | 95% relative activity of pure enzyme | Vigna radiata | GDP + alpha1-3 fucosylated oligosaccharide | - |
? | |
GDP-fucose + GnGnF6-peptide | 99% relative activity of pure enzyme and 70% relative activity of crude extract | Vigna radiata | GDP + alpha1-3-fucosylated GnGnF6-peptide | - |
? | |
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Vigna radiata | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? | |
additional information | the enzyme acts upon N-glycopeptides and related oligosaccharides with the glycan structure GlcNAc2Man3GlcNAc2, no transfer to N-glycans is observed when the terminal GlcNAc residues are either absent or substituted with galactose, N-acetyllactosamine, lacto-N-biose and N-acetylchito-oligosaccharides do not function as acceptors for the enzyme | Vigna radiata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Vigna radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Vigna radiata |