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Literature summary for 2.4.1.214 extracted from

  • Fabini, G.; Freilinger, A.; Altmann, F.; Wilson, I.B.H.
    Identification of core alpha1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster. Potential basis of the neural anti-horseradish peroxidase epitope (2001), J. Biol. Chem., 276, 28058-28067.
    View publication on PubMed
Search for more literature for 2.4.1.214

Cloned(Commentary)

Cloned (Comment) Organism
three genes FucTA, FucTB and FucTC encode proteins similar to alpha1-3-fucosyltransferases, expression in Pichia pastoris Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 dansyl-GnGnF6 glycopeptide derived from IgG, FucTA gene Drosophila melanogaster
0.046
-
 dansyl-GnGn glycopeptide derived from IgG, FucTA gene Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Drosophila melanogaster
-
 GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + dabsyl-GnGn-peptide GnGn is the most suitable substrate for FucTA Drosophila melanogaster GDP + alpha1-3-fucosylated dabsyl-GnGn-peptide
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 ?
GDP-fucose + dansyl-GnGn
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 Drosophila melanogaster GDP + alpha1-3-fucosylated glycopeptide
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 ?
GDP-fucose + dansyl-GnGnF6 prefucosylation of GnGn with chicken heart extract results in a superior conversion of GnGnF6 by FucTA than with GnGn, 100% relative conversion Drosophila melanogaster GDP + alpha1-3-fucosylated glycopeptide
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 ?
GDP-fucose + GalGal-N-glycan 4.6% relative conversion, FucTA gene Drosophila melanogaster GDP + alpha1-3-fucosylated GalGal-N-glycan
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 ?
GDP-fucose + GalGalF6-N-glycan 19.8% relative conversion, FucTA gene Drosophila melanogaster GDP + alpha1-3-fucosylated GalGalF6-N-glycan
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 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Drosophila melanogaster GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
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 ?
additional information the enzyme is able to fucosylate N-glycan structures of human transferrin in vitro Drosophila melanogaster ?
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additional information alpha1-6-fucosylated glycans are better substrates than beta-1-4-galactosylated glycans, FucTA gene Drosophila melanogaster ?
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 ?