Literature summary for 2.4.1.212 extracted from

Hubbard, C.; McNamara, J.T.; Azumaya, C.; Patel, M.S.; Zimmer, J.
The hyaluronan synthase catalyzes the synthesis and membrane translocation of hyaluronan (2012), J. Mol. Biol., 418, 21-31.

Search for more literature for 2.4.1.212

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally His6-tagged Se-HAS in Escherichia coli strain C43	Streptococcus dysgalactiae subsp. equisimilis

Protein Variants

Protein Variants	Comment	Organism
additional information	purified Se-HAS is reconstituted into proteoliposomes, from total lipid extract from Escherichia coli, where it synthesizes and translocates hyaluronan, or reconstituted into synthetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine/1,1',2,2'-tetraoleoyl cardiolipin/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (80/10/10%) proteoliposomes. In vitro synthesized, high-molecular-weight hyaluronan remains tightly associated with the intact proteoliposomes, even after proteolyticdegradation of HAS or in the presence of 1 M NaCl or 0.6 M urea to prevent nonspecific interactions of the polymer with the lipid vesicles	Streptococcus dysgalactiae subsp. equisimilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	the hyaluronan polymer is synthesized on the cytosolic side of the cell membrane by the membrane-embedded hyaluronan synthase	Streptococcus dysgalactiae subsp. equisimilis	5886	-

Organism

Organism	UniProt	Comment	Textmining
Streptococcus dysgalactiae subsp. equisimilis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia ccoli strain C43 by solubilization with detergent LysoFosCholine Ether-14, nickel affinity chromatography, and gel filtration	Streptococcus dysgalactiae subsp. equisimilis

Synonyms

Synonyms	Comment	Organism
HAS	-	Streptococcus dysgalactiae subsp. equisimilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Streptococcus dysgalactiae subsp. equisimilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Streptococcus dysgalactiae subsp. equisimilis

General Information

General Information	Comment	Organism
physiological function	the hyaluronan synthase catalyzes the synthesis and membrane translocation of hyaluronan, it is both necessary and sufficient to translocate hyaluronan in a reaction that is tightly coupled to hyaluronan elongation. Hyaluronan synthesis and translocation are spatially coupled events, which allow hyaluronan synthesis even in the presence of a large excess of hyaluronan-degrading enzyme	Streptococcus dysgalactiae subsp. equisimilis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)