Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.211 extracted from

  • Hidaka, M.; Nishimoto, M.; Kitaoka, M.; Wakagi, T.; Shoun, H.; Fushinobu, S.
    The crystal structure of galacto-N-biose/lacto-N-biose I phosphorylase: a large deformation of a TIM barrel scaffold (2009), J. Biol. Chem., 284, 7273-7283.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the non-labeled enzyme is expressed in Escherichia coli BL21(DE3) cells, the selenomethionine-labeled enzyme is expressed in Escherichia coli strain B834 (DE3) Bifidobacterium longum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium cacodylate (pH 6.5), 0.2 M Mg(NO3)2, and 15% (v/v) polyethylene glycol 4000 Bifidobacterium longum

Protein Variants

Protein Variants Comment Organism
D313N mutant with undetectable activity Bifidobacterium longum
F364N mutant shows severely impaired activity Bifidobacterium longum
N166A mutant shows severely impaired activity Bifidobacterium longum
R210E the mutant shows no detectable activity Bifidobacterium longum
R32E the mutant shows no detectable activity Bifidobacterium longum
R358E the mutant shows no detectable activity Bifidobacterium longum
Y362F the mutation results in about 1000fold reduction of the catalytic efficiency Bifidobacterium longum
Y362N the mutation results in the complete loss of the activity Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.72
-
alpha-D-galactose 1-phosphate wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
17
-
alpha-D-galactose 1-phosphate mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
19
-
alpha-D-galactose 1-phosphate mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
24
-
alpha-D-galactose 1-phosphate mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum E8MF13
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bifidobacterium longum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
-
Bifidobacterium longum alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Bifidobacterium longum alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
Gal-HexNAcP
-
Bifidobacterium longum
galacto-N-biose/lacto-N-biose I phosphorylase
-
Bifidobacterium longum
GLNBP
-
Bifidobacterium longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
alpha-D-galactose 1-phosphate mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
0.3
-
alpha-D-galactose 1-phosphate mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
4.83
-
alpha-D-galactose 1-phosphate mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
10.7
-
alpha-D-galactose 1-phosphate wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum

General Information

General Information Comment Organism
physiological function key enzyme for intestinal growth, phosphorolyses galacto-N-biose and lacto-N-biose I with anomeric inversion Bifidobacterium longum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
alpha-D-galactose 1-phosphate mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
0.012
-
alpha-D-galactose 1-phosphate mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
0.26
-
alpha-D-galactose 1-phosphate mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
6.21
-
alpha-D-galactose 1-phosphate wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum