Cloned (Comment) | Organism |
---|---|
the non-labeled enzyme is expressed in Escherichia coli BL21(DE3) cells, the selenomethionine-labeled enzyme is expressed in Escherichia coli strain B834 (DE3) | Bifidobacterium longum |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M sodium cacodylate (pH 6.5), 0.2 M Mg(NO3)2, and 15% (v/v) polyethylene glycol 4000 | Bifidobacterium longum |
Protein Variants | Comment | Organism |
---|---|---|
D313N | mutant with undetectable activity | Bifidobacterium longum |
F364N | mutant shows severely impaired activity | Bifidobacterium longum |
N166A | mutant shows severely impaired activity | Bifidobacterium longum |
R210E | the mutant shows no detectable activity | Bifidobacterium longum |
R32E | the mutant shows no detectable activity | Bifidobacterium longum |
R358E | the mutant shows no detectable activity | Bifidobacterium longum |
Y362F | the mutation results in about 1000fold reduction of the catalytic efficiency | Bifidobacterium longum |
Y362N | the mutation results in the complete loss of the activity | Bifidobacterium longum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.72 | - |
alpha-D-galactose 1-phosphate | wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
17 | - |
alpha-D-galactose 1-phosphate | mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
19 | - |
alpha-D-galactose 1-phosphate | mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
24 | - |
alpha-D-galactose 1-phosphate | mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum | E8MF13 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bifidobacterium longum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | - |
Bifidobacterium longum | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Bifidobacterium longum | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gal-HexNAcP | - |
Bifidobacterium longum |
galacto-N-biose/lacto-N-biose I phosphorylase | - |
Bifidobacterium longum |
GLNBP | - |
Bifidobacterium longum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
alpha-D-galactose 1-phosphate | mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
0.3 | - |
alpha-D-galactose 1-phosphate | mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
4.83 | - |
alpha-D-galactose 1-phosphate | mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
10.7 | - |
alpha-D-galactose 1-phosphate | wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum |
General Information | Comment | Organism |
---|---|---|
physiological function | key enzyme for intestinal growth, phosphorolyses galacto-N-biose and lacto-N-biose I with anomeric inversion | Bifidobacterium longum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
alpha-D-galactose 1-phosphate | mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
0.012 | - |
alpha-D-galactose 1-phosphate | mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
0.26 | - |
alpha-D-galactose 1-phosphate | mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum | |
6.21 | - |
alpha-D-galactose 1-phosphate | wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate | Bifidobacterium longum |