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Literature summary for 2.4.1.211 extracted from

  • Derensy-Dron, D.; Krzewinski, F.; Brassart, C.; Bouquelet, S.
    beta-1,3-Galactosyl-N-acetylhexosamine phosphorylase from Bifidobacterium bifidum DSM 20082: characterization, partial purification and relation to mucin degradation (1999), Biotechnol. Appl. Biochem., 29, 3-10.
    View publication on PubMed

Application

Application Comment Organism
medicine galactosyl-N-acetylhexosamine phosphorylase has a predominant role in the digestive tract of human and in the metabolism of galactose Bifidobacterium bifidum
nutrition dairy industry production of different fermented bifidobacteria milks Bifidobacterium bifidum
synthesis production of dissaccharidic structures instead of chemical synthesis Bifidobacterium bifidum

General Stability

General Stability Organism
unstable after cell lysis Bifidobacterium bifidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
alpha-D-galactose 1-phosphate
-
Bifidobacterium bifidum
2.7
-
phosphate
-
Bifidobacterium bifidum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Bifidobacterium bifidum 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
gel filtration Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
-
DSM 20082
-

Purification (Commentary)

Purification (Comment) Organism
partly Bifidobacterium bifidum

Reaction

Reaction Comment Organism Reaction ID
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine + phosphate = alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine reaction also occurs with beta-D-galactopyranosyl-(1->3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
Bifidobacterium bifidum lacto-N-biose + phosphate
-
?
beta D-galactosido-(1,3)-N-acetylgalactosamine + H2O
-
Bifidobacterium bifidum alpha-D-galactose-1-phosphate + GalNAc
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosoamine + phosphate hydrolysis involves a Walden inversion Bifidobacterium bifidum alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55 phosphorolysis and reverse reaction Bifidobacterium bifidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60
-
Bifidobacterium bifidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 reverse reaction Bifidobacterium bifidum
6 6.5 galactophosphorolytic activity Bifidobacterium bifidum