Application | Comment | Organism |
---|---|---|
medicine | galactosyl-N-acetylhexosamine phosphorylase has a predominant role in the digestive tract of human and in the metabolism of galactose | Bifidobacterium bifidum |
nutrition | dairy industry production of different fermented bifidobacteria milks | Bifidobacterium bifidum |
synthesis | production of dissaccharidic structures instead of chemical synthesis | Bifidobacterium bifidum |
General Stability | Organism |
---|---|
unstable after cell lysis | Bifidobacterium bifidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
alpha-D-galactose 1-phosphate | - |
Bifidobacterium bifidum | |
2.7 | - |
phosphate | - |
Bifidobacterium bifidum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Bifidobacterium bifidum | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration | Bifidobacterium bifidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
DSM 20082 | - |
Purification (Comment) | Organism |
---|---|
partly | Bifidobacterium bifidum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine + phosphate = alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine | reaction also occurs with beta-D-galactopyranosyl-(1->3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product | Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
Bifidobacterium bifidum | lacto-N-biose + phosphate | - |
? | |
beta D-galactosido-(1,3)-N-acetylgalactosamine + H2O | - |
Bifidobacterium bifidum | alpha-D-galactose-1-phosphate + GalNAc | - |
r | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosoamine + phosphate | hydrolysis involves a Walden inversion | Bifidobacterium bifidum | alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | phosphorolysis and reverse reaction | Bifidobacterium bifidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | - |
Bifidobacterium bifidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | reverse reaction | Bifidobacterium bifidum |
6 | 6.5 | galactophosphorolytic activity | Bifidobacterium bifidum |