Application | Comment | Organism |
---|---|---|
food industry | Limonoid glucosyltransferase is an enzyme that catalyzes the conversion of bitter limonoid into non-bitter limonoid glucoside while retaining the health benefit of limonoids in the juice. The immobilization of this enzyme in a column can solve the juice bitterness problem | Citrus maxima x Citrus paradisi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | modifies tryptophan residues | Citrus maxima x Citrus paradisi | |
3-nitro-L-tyrosine ethylester | modifies tryptophan residues | Citrus maxima x Citrus paradisi | |
citraconic anhydride | modifies lysine residues | Citrus maxima x Citrus paradisi | |
diethyldicarbonate | modifies histidine residues and inactivates the enzyme, pseudo first order kinetics. When the histidine modification is reversed by hydroxylamine treatment, 79% of the activity is restored | Citrus maxima x Citrus paradisi | |
additional information | inactivation of the enzyme following modification of carboxyl and imidazole moieties is a consequence of a loss in substrate binding and catalysis in the glucosyltransfer reaction. No inhibition by serine modifiying diisopropyl fluorophosphate and cysteine modifying 4-chloromercuribenzoate and iodoacetamide | Citrus maxima x Citrus paradisi | |
N-bromosuccinimide | modifies tryptophan residues and inactivates the enzyme, loss of LGTase activity by NBS treatment is partially protected by pre-incubating the enzyme with excess limonin | Citrus maxima x Citrus paradisi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
limonin | pH 7.0-8.4, 25°C | Citrus maxima x Citrus paradisi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + limonin | Citrus maxima x Citrus paradisi | - |
glucosyl-limonin + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus maxima x Citrus paradisi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + limonin + H2O = glucosyl-limonin + UDP | catalytic reaction mechanism, overview | Citrus maxima x Citrus paradisi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + limonin | - |
Citrus maxima x Citrus paradisi | glucosyl-limonin + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LGTase | - |
Citrus maxima x Citrus paradisi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Citrus maxima x Citrus paradisi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
91.7 | - |
limonin | pH 7.0-8.4, 25°C | Citrus maxima x Citrus paradisi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Citrus maxima x Citrus paradisi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at an acidic pH the D-ring of the substrate that should be in an open form is closed and glycosylation does not occur. The decrease in activity at acidic pH also indicates that an essential residue participates in catalytic functioning while in the unprotonated state | Citrus maxima x Citrus paradisi |
5.5 | 9.5 | activity range | Citrus maxima x Citrus paradisi |
General Information | Comment | Organism |
---|---|---|
additional information | limonoid glucosyltransferase is an enzyme that catalyzes the conversion of bitter limonoid into non-bitter limonoid glucoside while retaining the health benefit of limonoids in the juice | Citrus maxima x Citrus paradisi |