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mutations that eliminate any one of starch synthases SSIII, SSIIa, and starch branching enzymes SBEIIa, and SBEIIb also prevent the others from assembling into a high molecular mass form of approximately 670 kDa. SSIII, SSIIa, SBEIIa, and SBEIIb most likely all exist together in the same complex. SSIIa, SBEIIb, and SBEIIa, but not SSIII, are also interdependent for assembly into a complex of approximately 300 kDa. SSIII, SSIIa, SBEIIa, and SBEIIb copurifiy through successive chromatography steps, and SBEIIa, SBEIIb, and SSIIa coimmunoprecipitate with SSIII in a phosphorylation-dependent manner. Pyruvate orthophosphate dikinase and sucrose synthase isoform SUS-SH1 require SSIII, SSIIa, SBEIIa, and SBEIIb for assembly into the 670 kDa complex |
Zea mays |