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Literature summary for 2.4.1.21 extracted from
- Properties of primer-dependent starch synthesis catalysed by starch synthase from potato tubers (1990), Phytochemistry, 29, 719-723.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
ADPglucose | - |
Solanum tuberosum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Solanum tuberosum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
possibly a monomer of 70000 Da or a dimer of 2 * 38000 Da | Solanum tuberosum |
| 70000 | - |
gel filtration | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
potato | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, maltotriose-Sepharose, DEAE-Toyopearl, Sepharose Cl-6B | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.026 | - |
in the presence of 0.5 mg glycogen primer and 500 mM sodium citrate | Solanum tuberosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 4 months, no loss of activity | Solanum tuberosum |
| 4°C, at least 2 weeks, no loss of activity | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + alpha-1,4-polyglucan | citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen | Solanum tuberosum | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptor: amylose | Solanum tuberosum | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptor: amylopectin | Solanum tuberosum | ADP + alpha-1,4-polyglucan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | possibly a monomer of 70000 Da or a dimer of 2 * 38000 Da | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Solanum tuberosum |
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Release 2023.1 (January 2023)
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