Cloned (Comment) | Organism |
---|---|
gene XTH15, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted | Arabidopsis thaliana |
gene XTH31, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | classical hyperbolic Michaelis-Menten curve for XET in the case of XTH15 | Arabidopsis thaliana | |
additional information | - |
additional information | Km of the XET activity of XTH31 for tamarind xyloglucan cannot be estimated because a hyperbola is not obtained | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P93046 | - |
- |
Arabidopsis thaliana | Q38911 | - |
- |
no activity in Pichia pastoris | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels) | Arabidopsis thaliana | - |
additional information | enzyme XTH31 is strongly expressed in young, rapidly growing organs | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro | Arabidopsis thaliana | ? | - |
? | |
tamarind xyloglucan + XXXG | the enzyme performs transglucosylation | Arabidopsis thaliana | ? | - |
? | |
tamarind xyloglucan + XXXGol | the enzyme performs transglucosylation | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | see also EC 3.2.1.151 | Arabidopsis thaliana |
XET | - |
Arabidopsis thaliana |
XTH15 | - |
Arabidopsis thaliana |
XTH31 | - |
Arabidopsis thaliana |
xyloglucan endotransglucosylase | - |
Arabidopsis thaliana |
xyloglucan endotransglucosylase/hydrolase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | a role for enzyme XTH31 in acid growth. Isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity | Arabidopsis thaliana |
physiological function | isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity | Arabidopsis thaliana |