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Literature summary for 2.4.1.207 extracted from

  • Shi, Y.; Zhu, X.; Miller, J.; Gregson, T.; Zheng, S.; Fry, S.
    Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia (2015), Phytochemistry, 112, 160-169 .
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Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
gene XTH15, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted Arabidopsis thaliana
gene XTH31, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information classical hyperbolic Michaelis-Menten curve for XET in the case of XTH15 Arabidopsis thaliana
additional information
-
 additional information Km of the XET activity of XTH31 for tamarind xyloglucan cannot be estimated because a hyperbola is not obtained Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P93046
-
-
Arabidopsis thaliana Q38911
-
-
no activity in Pichia pastoris
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels) Arabidopsis thaliana
-
additional information enzyme XTH31 is strongly expressed in young, rapidly growing organs Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro Arabidopsis thaliana ?
-
 ?
tamarind xyloglucan + XXXG the enzyme performs transglucosylation Arabidopsis thaliana ?
-
 ?
tamarind xyloglucan + XXXGol the enzyme performs transglucosylation Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
More see also EC 3.2.1.151 Arabidopsis thaliana
XET
-
 Arabidopsis thaliana
XTH15
-
 Arabidopsis thaliana
XTH31
-
 Arabidopsis thaliana
xyloglucan endotransglucosylase
-
 Arabidopsis thaliana
xyloglucan endotransglucosylase/hydrolase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function a role for enzyme XTH31 in acid growth. Isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity Arabidopsis thaliana
physiological function isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity Arabidopsis thaliana