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Literature summary for 2.4.1.207 extracted from
- Isolation and characterization of two persimmon xyloglucan endotransglycosylase/hydrolase (XTH) genes that have divergent functions in cell wall modification and fruit postharvest softening (2016), Front. Plant Sci., 7, 624 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene XTH6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells. Recombinant expression of DkXTH6 without the signal peptide in Escherichia coli strain BL21 | Diospyros kaki |
| gene XTH7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of DkXTH7 without the signal peptide in Escherichia coli strain BL21 | Diospyros kaki |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Diospyros kaki | 5618 | - |
| extracellular | - |
Diospyros kaki | - |
- |
| additional information | the enzyme contains a signal peptide | Diospyros kaki | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diospyros kaki | A0A067XR63 | cv. Fupingjianshi, i.e. Diospyros chinensis | - |
| Diospyros kaki | A0A067XRK9 | cv. Fupingjianshi, i.e. Diospyros chinensis | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains a potential N-linked glycosylation (N-X-S/T) site | Diospyros kaki |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Diospyros kaki | - |
| flower | low expression level | Diospyros kaki | - |
| fruit | low expression level in young fruits, very low expression level in ripe fruits | Diospyros kaki | - |
| fruit | lower expression level in young fruits, moderate to high expression level in ripe fruits | Diospyros kaki | - |
| leaf | high expression level in young leaves, very high in older leaves | Diospyros kaki | - |
| leaf | very high expression level in young leaves, low in older leaves | Diospyros kaki | - |
| stem | - |
Diospyros kaki | - |
| stem | lower expression level | Diospyros kaki | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30830, sequence calculation | Diospyros kaki |
| ? | x * 33760, sequence calculation | Diospyros kaki |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DkXTH6 | - |
Diospyros kaki |
| DkXTH7 | - |
Diospyros kaki |
| More | cf. EC 3.2.1.151 | Diospyros kaki |
| XEH | - |
Diospyros kaki |
| XTH6 | - |
Diospyros kaki |
| XTH7 | - |
Diospyros kaki |
| xyloglucan endohydrolase | - |
Diospyros kaki |
| xyloglucan endotransglycosylase/hydrolase | - |
Diospyros kaki |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant enzyme | Diospyros kaki |
| 6 | - |
recombinant enzyme | Diospyros kaki |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | recombinant enzyme, activity range, profile overview | Diospyros kaki |
| 3 | 8 | recombinant enzyme, activity range, profile overview | Diospyros kaki |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Diospyros kaki | sequence calculation | - |
5.48 |
| Diospyros kaki | sequence calculation | - |
6.32 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Diospyros kaki | induced by propylene and abscisic acid | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | evolutionary relationship of persimmon DkXTH6 and DkXTH7 genes among other plant species and phylogenetic tree, overview. DkXTH6 and DkXTH7 possess several functional domains typical in plant XTHs, including the conserved amino acids (DEIDFEFLG) as a putative active site and together with a potential N-linked glycosylation (N-X-S/T) site | Diospyros kaki |
| physiological function | XTH, an important enzyme involved in xyloglucan metabolism, can function as a xyloglucan endotransglycosylase (XET, EC 2.4.1.207) and/or a xyloglucan endohydrolase (XEH, EC 3.2.1.151), with the former transferring one xyloglucan molecule fragment to another and the latter responsible for hydrolysis of one xyloglucan molecule. DkXTH7 is likely to be involved in cell wall assembly, special roles of isozyme XTH6 and XTH7 in persimmon fruit softening, overvie. The XTH gene family members each play a certain role in plant growth, fruit ripening, and fruit softening. During persimmon fruit postharvest softening,expression of isozymes DkXTH6 and DkXTH7 follows two opposing patterns | Diospyros kaki |
| physiological function | XTH, an important enzyme involved in xyloglucan metabolism, can function as a xyloglucan endotransglycosylase (XET, EC 2.4.1.207) and/or a xyloglucan endohydrolase (XEH, EC 3.2.1.151), with the former transferring one xyloglucan molecule fragment to another and the latter responsible for hydrolysis of one xyloglucan molecule. Enzyme DkXTH6 might take part in cell wall restructuring, special roles of isozyme XTH6 and XTH7 in persimmon fruit softening, overview. The XTH gene family members each play a certain role in plant growth, fruit ripening, and fruit softening. During persimmon fruit postharvest softening,expression of isozymes DkXTH6 and DkXTH7 follows two opposing patterns | Diospyros kaki |
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