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Literature summary for 2.4.1.207 extracted from
- Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening (2015), PLoS ONE, 10, e0123668.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DkXTH1, DNA and amino acid sequence determination and analysis, sequence comparison of isozymes and phylogenetic analysis, quantitative real-time PCR expression analysis, mature coding region of DkXTH1 is expressed in Escherichia coli strain BL21, recombinant expression of GFP-tagged isozyme lacking the sugnal peptide and of the DkXTH1 signal peptide fused to GFP in onion cells | Diospyros kaki |
| gene DkXTH2, DNA and amino acid sequence determination and analysis, sequence comparison of isozymes and phylogenetic analysis, quantitative real-time PCR expression analysis, the isozyme is expressed without the N-terminal leader sequence in Escherichia coli strain BL21, recombinant expression of full-length GFP-tagged isozyme in onion cells | Diospyros kaki |
| gene DkXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21 | Diospyros kaki |
| gene DkXTH4, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, sequence comparison of isozymes and phylogenetic analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21 | Diospyros kaki |
| gene DkXTH5, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, sequence comparison of isozymes and phylogenetic analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21 | Diospyros kaki |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits | Diospyros kaki |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Diospyros kaki | 5618 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diospyros kaki | A0A067XSJ2 | cv. Fuping Jianshi, cv. Ganmaokui | - |
| Diospyros kaki | G5DAC6 | cv. Fuping Jianshi, cv. Ganmaokui | - |
| Diospyros kaki | G5DAC7 | cv. Fuping Jianshi, cv. Ganmaokui | - |
| Diospyros kaki | G5DAC8 | cv. Fuping Jianshi, cv. Ganmaokui | - |
| Diospyros kaki | G5DAC9 | cv. Fuping Jianshi, cv. Ganmaokui | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozyme DkXTH3 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration | Diospyros kaki |
| recombinant His-tagged isozyme DkXTH4 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration | Diospyros kaki |
| recombinant His-tagged isozyme DkXTH5 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration | Diospyros kaki |
| recombinant His-tagged isozyme without the N-terminal leader sequence from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration | Diospyros kaki |
| recombinant His-tagged mature coding region of isozyme DkXTH1 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration | Diospyros kaki |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | isozyme DkXTH2 reaches maximum expression concomitance with pronounced fruit softening | Diospyros kaki | - |
| fruit | isozyme DkXTH3 reaches maximum expression concomitance with pronounced fruit softening | Diospyros kaki | - |
| fruit | the expression of isozyme DkXTH1 is very high in immature growing fruit and peaks before the mature stage | Diospyros kaki | - |
| fruit | the expression of isozyme DkXTH4 is very high in immature growing fruit and peaks before the mature stage | Diospyros kaki | - |
| fruit | the expression of isozyme DkXTH5 is very high in immature growing fruit and peaks before the mature stage | Diospyros kaki | - |
| additional information | isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview | Diospyros kaki | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-dimensional structure of isozyme DkXTH1 | Diospyros kaki |
| More | three-dimensional structure of isozyme DkXTH2 | Diospyros kaki |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XTH1 | - |
Diospyros kaki |
| XTH2 | - |
Diospyros kaki |
| XTH3 | - |
Diospyros kaki |
| XTH4 | - |
Diospyros kaki |
| XTH5 | - |
Diospyros kaki |
| xyloglucan endotransglycosylase/hydrolase | - |
Diospyros kaki |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | recombinant truncated isozyme | Diospyros kaki |
| 5 | 5.5 | recombinant truncated isozyme | Diospyros kaki |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Diospyros kaki | sequence calculation | - |
5.36 |
| Diospyros kaki | sequence calculation | - |
6.45 |
| Diospyros kaki | sequence calculation | - |
8.66 |
| Diospyros kaki | sequence calculation | - |
8.94 |
| Diospyros kaki | sequence calculation | - |
9.28 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Diospyros kaki | the expression levels of isozyme DkXTH1 in Fuping Jianshi persimmon fruit is markedly induced by cold treatment, and it is also higher in the firmer persimmon cultivar Ganmaokui compared with those in the softer cultivar (Fuping Jianshi) during storage at 25°C | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | xyloglucan endotransglycosylase/hydrolase enzymes plays a role in the remodeling of cell wall hemicelluloses. Function of isozymes in persimmon fruit development and postharvest softening. DkXTH isozymes function by targeting to the cell wall under regulation of a signal peptide | Diospyros kaki |
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