Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.207 extracted from

  • Chormova, D.; Franková, L.; Defries, A.; Cutler, S.; Fry, S.
    Discovery of small molecule inhibitors of xyloglucan endotransglucosylase (XET) activity by high-throughput screening (2015), Phytochemistry, 117, 220-236.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.207

Inhibitors

Inhibitors Comment Organism Structure
(-)-epicatechin gallate
-
 Petroselinum sp.
(-)-epigallocatechin gallate non-specific Petroselinum sp.
1-amino-4-hydroxy-2-methoxyanthracene-9,10-dione
-
 Petroselinum sp.
3-([3-(4-hydroxyphenyl)-3-oxopropanoyl]amino)-4-[methyl(octadecyl)amino]-benzoic acid
-
 Petroselinum sp.
4-([(4-methylphenyl)thio]methyl)-N-(2-pyridinylmethyl)benzamide
-
 Petroselinum sp.
4-chloromercuribenzoate
-
 Petroselinum sp.
6-bromo-2-hydroxy-1Hbenzo[de]isoquinoline-1,3-(2H)-dione
-
 Petroselinum sp.
aniline blue
-
 Petroselinum sp.
baicalein
-
 Petroselinum sp.
brilliant blue G specific inhibition Petroselinum sp.
Bromocresol green
-
 Petroselinum sp.
bromocresol purple
-
 Petroselinum sp.
ebselen
-
 Petroselinum sp.
eosin
-
 Petroselinum sp.
erythrosin
-
 Petroselinum sp.
hesperetin
-
 Petroselinum sp.
luteolin
-
 Petroselinum sp.
m-digallic acid
-
 Petroselinum sp.
additional information high-throughput fluorescent dot-blot screening to search for enzyme inhibitors in vitro, detailed overview. Of 4216 xenobiotics tested, with cellulose-bound xyloglucan as donor-substrate, 18 inhibit XET activity and 18 promote it (especially anthraquinones and flavonoids). No compounds promotes XET in quantitative assays with (cellulose-free) soluble xyloglucan as substrate, suggesting that promotion is dependent on enzyme-cellulose interactions. With cellulose-free xyloglucan as substrate, 22 XET-inhibitors are identified, especially compounds that generate singlet oxygen. Inhibitors include tannins, sulphydryl reagent,s and triphenylmethanes Petroselinum sp.
Mycophenolic acid
-
 Petroselinum sp.
N'-(1-E)-(5-bromofuran-2-yl)-methylidene-2-(3-methylphenoxy)-acetohydrazide
-
 Petroselinum sp.
Phenolphthalein
-
 Petroselinum sp.
phenylmercuric acetate
-
 Petroselinum sp.
phloretin
-
 Petroselinum sp.
retinoic acid
-
 Petroselinum sp.
riboflavin the riboflavin inhibitory effect is light-dependent, non-specific Petroselinum sp.
Tannic acid
-
 Petroselinum sp.
thiomersal
-
 Petroselinum sp.
vulpinic acid specific inhibition Petroselinum sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Petroselinum sp. 5618
-

Organism

Organism UniProt Comment Textmining
Petroselinum sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information usage of cellulose-bound tamarind seed xyloglucan as donor substrate and [1-3H]XXXGol as acceptor substrate Petroselinum sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
XET
-
 Petroselinum sp.
xyloglucan endotransglucosylase
-
 Petroselinum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Petroselinum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Petroselinum sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.027
-
 pH 5.5, 20°C Petroselinum sp. eosin
0.029
-
 pH 5.5, 20°C Petroselinum sp. riboflavin
0.036
-
 pH 5.5, 20°C Petroselinum sp. erythrosin
0.082
-
 pH 5.5, 20°C Petroselinum sp. Tannic acid
0.15
-
 pH 5.5, 20°C Petroselinum sp. aniline blue
0.27
-
 pH 5.5, 20°C Petroselinum sp. Bromocresol green
0.31
-
 pH 5.5, 20°C Petroselinum sp. 4-chloromercuribenzoate
0.38
-
 pH 5.5, 20°C Petroselinum sp. (-)-epigallocatechin gallate
0.43
-
 pH 5.5, 20°C Petroselinum sp. (-)-epicatechin gallate
0.47
-
 pH 5.5, 20°C Petroselinum sp. m-digallic acid
0.52
-
 pH 5.5, 20°C Petroselinum sp. baicalein
0.52
-
 pH 5.5, 20°C Petroselinum sp. Phenolphthalein
0.58
-
 pH 5.5, 20°C Petroselinum sp. phloretin
0.61
-
 pH 5.5, 20°C Petroselinum sp. hesperetin
0.62
-
 pH 5.5, 20°C Petroselinum sp. vulpinic acid
0.7
-
 pH 5.5, 20°C Petroselinum sp. luteolin

General Information

General Information Comment Organism
evolution xyloglucan endotransglucosylase (XET) is a homotransglycanase enzyme activity found in all land plants and in some charophytic algae Petroselinum sp.