Cloned (Comment) | Organism |
---|---|
into the pBIm and pCAM-BIA1300 vector, and into the pPIC6A expression vector for the production of recombinant AtXTH21 protein using the Pichia pastoris expression system | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | purified AtXTH21 activity, Km: 0.6245 mg/ml | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
determined by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9ZV40 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan | Arabidopsis thaliana | ? | - |
? | |
tamarind xyloglucan + XLLG-9-aminopyrene-1,4,6-trisulfonate | XET activity assay | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtXTH21 | - |
Arabidopsis thaliana |
XET | - |
Arabidopsis thaliana |
XTH | - |
Arabidopsis thaliana |
xyloglucan endotransglucosylase/hydrolase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
XET activity assay | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
XET activity assay | Arabidopsis thaliana |