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Literature summary for 2.4.1.207 extracted from

  • Liu, Y.B.; Lu, S.M.; Zhang, J.F.; Liu, S.; Lu, Y.T.
    A xyloglucan endotransglucosylase/hydrolase involves in growth of primary root and alters the deposition of cellulose in Arabidopsis (2007), Planta, 226, 1547-1560.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pBIm and pCAM-BIA1300 vector, and into the pPIC6A expression vector for the production of recombinant AtXTH21 protein using the Pichia pastoris expression system Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information purified AtXTH21 activity, Km: 0.6245 mg/ml Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
determined by SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9ZV40
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan Arabidopsis thaliana ?
-
?
tamarind xyloglucan + XLLG-9-aminopyrene-1,4,6-trisulfonate XET activity assay Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
AtXTH21
-
Arabidopsis thaliana
XET
-
Arabidopsis thaliana
XTH
-
Arabidopsis thaliana
xyloglucan endotransglucosylase/hydrolase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
XET activity assay Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
XET activity assay Arabidopsis thaliana