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Literature summary for 2.4.1.207 extracted from
- A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans (2007), J. Biol. Chem., 282, 12951-12962.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | inhibits activity by 3-4% at 5-15 mM | Hordeum vulgare |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity by 7-8% at 5-15 mM | Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
SDS-PAGE | Hordeum vulgare |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | the addition of 10% (v/v) glycerol has no affect on XET activity after several freeze-thawing cycles | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
cultivar Clipper | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography, chromatofocusing on PBE-94, and Bio-Gel P-60 gel filtration | Hordeum vulgare |
| Sepharose Q column chromatography, Phenyl-Sepharose chromatography, PBE-94 column chromatography and Bio-Gel P-60 gel filtration | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000000072 | - |
enzyme from crude homogenate, at 30°C | Hordeum vulgare |
| 0.0002178 | - |
after 3025fold purification, at 30°C | Hordeum vulgare |
| 0.02 | - |
crude cell extract, at 30°C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride | Hordeum vulgare |
| 60.51 | - |
after 3025fold purification, at 30°C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride | Hordeum vulgare |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 100 mM succinate or ammonium acetate buffers at pH 6.0 containing 5 mM calcium chloride, 1 year, no loss of activity | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor barley (1,3,1,4)-beta-D-glucan + acceptor cellooligosaccharide | 0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor barley (1,3,1,4)-beta-D-glucan + acceptor xyloglucan oligosaccharide | 0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor carboxymethylcellulose + acceptor xyloglucan oligosaccharide | 0.4% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor hydroxyethylcellulose + acceptor cellooligosaccharide | 0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor hydroxyethylcellulose + acceptor xyloglucan oligosaccharide | 44.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor locust bean gum + acceptor xyloglucan oligosaccharide | 0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor sulfuric acid-swollen cellulose + acceptor xyloglucan oligosaccharide | 5% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor tamarind xyloglucan + acceptor cellooligosaccharide | 0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide | Hordeum vulgare | ? | - |
? | |
| donor tamarind xyloglucan + acceptor xyloglucan oligosaccharide | - |
Hordeum vulgare | ? | - |
? | |
| additional information | no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin, and esterified pectin (potassium salt), orange polygalactouronic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, beta-D-galactans, and arabinogalactan protein with xyloglucan oligosaccharide and cellooligosaccharide, no transferase activity is detected laminari-oligosaccharides as acceptor substrates | Hordeum vulgare | ? | - |
? | |
| additional information | no transferase activity is detected with laminarin, lichenin, pustulan, barley arabinoxylan, glucomannan, citrus fruit pectin or esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, lupin or potato beta-D-galactans, or gum arabic | Hordeum vulgare | ? | - |
? | |
| additional information | XET5 catalyzes the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates and between xyloglucans and (1,3 1,4)-beta-D-glucans | Hordeum vulgare | ? | - |
? | |
| xyloglucan + barley (1,3 1,4)-beta-D-glucan | the rate of covalent bond formation with barley (1,3 1,4)-beta-D-glucan is significant but slower compared with that on tamarind xyloglucan | Hordeum vulgare | ? | - |
? | |
| xyloglucan + heptaitol | - |
Hordeum vulgare | ? | - |
? | |
| xyloglucan + hydroxyethylcellulose | the rate of covalent bond formation with hyrdoxyethylcellulose is comparable with that on tamarind xyloglucan | Hordeum vulgare | ? | - |
? | |
| xyloglucan + sulfuric acid-swollen cellulose | - |
Hordeum vulgare | ? | - |
? | |
| xyloglucan + tamarind xyloglucan | - |
Hordeum vulgare | ? | - |
? | |
| xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc | - |
Hordeum vulgare | ? | - |
? | |
| xyloglucan + Xylalpha(1,6)Glc-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc | - |
Hordeum vulgare | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XET5 | - |
Hordeum vulgare |
| xyloglucan endo-transglucosylase | - |
Hordeum vulgare |
| xyloglucan xyloglucosyl transferase | - |
Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | 30 | - |
Hordeum vulgare |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
the enzyme also operates efficiently at 0 °C, where it shows almost 40% of the activity observed at 30°C | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Hordeum vulgare |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | isoelectric focusing | - |
8.5 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | xyloglucan endo-transglucosylase can link different polysaccharides in vivo and hence influence cell wall strength, flexibility, and porosity | Hordeum vulgare |
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Release 2023.1 (January 2023)
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