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Literature summary for 2.4.1.206 extracted from
- Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T5), an essential enzyme for the expression of HNK-1 and lewis X epitopes on glycolipids (2001), J. Biol. Chem., 276, 22032-22040.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| retinoic acid | up-regulation of enzyme expression | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| DNA sequence determination and analysis | Homo sapiens |
| expression in Nawalma cells, membrane-bound | Homo sapiens |
| partial sequence, not a full length clone, DNA sequence determination | Rattus norvegicus |
| soluble expression as FLAG-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 12-O-tetradecanoylphorbol-13-acetate | down-regulation of enzyme expression | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BYG0 | - |
- |
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | Q99NB2 | not a full length clone | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant FLAG-tagged fusion protein from Sf21 insect cells | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| brain | low content | Homo sapiens | - |
| cerebellum | - |
Homo sapiens | - |
| cerebellum | - |
Rattus norvegicus | - |
| cerebral cortex | fetal | Rattus norvegicus | - |
| cerebral cortex | not adult | Rattus norvegicus | - |
| colon | - |
Homo sapiens | - |
| colonic adenocarcinoma cell | - |
Homo sapiens | - |
| hypophysis | - |
Homo sapiens | - |
| liver | very low content | Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| lymph node | low content | Homo sapiens | - |
| additional information | transcript content and activity in diverse tumour cell lines, overview, nearly undetectable content in leukemic cell lines | Homo sapiens | - |
| additional information | enzyme levels in brain regions during pre- and postnatal brain development | Rattus norvegicus | - |
| placenta | - |
Homo sapiens | - |
| spleen | low content | Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| thymus | low content | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity in diverse tumour cell lines, overview | Homo sapiens |
| additional information | - |
activity during brain development | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids | Rattus norvegicus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide | - |
Rattus norvegicus | UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
? | |
| UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide | i.e. lactosylceramide | Homo sapiens | UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
? | |
| UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide | acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine | Homo sapiens | UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
? | |
| UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine | Homo sapiens | UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta3Gn-T5 | - |
Homo sapiens |
| beta3Gn-T5 | - |
Rattus norvegicus |
| lactotriaosylceramide synthase | - |
Homo sapiens |
| lactotriaosylceramide synthase | - |
Rattus norvegicus |
| UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase | - |
Homo sapiens |
| UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
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