Literature summary for 2.4.1.201 extracted from

Taguchi, T.; Ogawa, T.; Kitajima, K.; Inoue, S.; Inoue, Y.; Ihara, Y.; Sakamoto, Y.; Nagai, K.; Taniguchi, N.
A method for determination of UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI activity using a pyridylaminated tetraantennary oligosaccharide as an acceptor substrate (1998), Anal. Biochem., 255, 155-157.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Triton X-100	-	Gallus gallus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	complete inhibition at 1 mM	Gallus gallus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Gallus gallus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	can substitute partially for Mn2+	Gallus gallus
Co2+	86% activity compared to Mn2+	Gallus gallus
Mg2+	can fully substitute for Mn2+, 25 mM	Gallus gallus
Mn2+	absolute requirement	Gallus gallus
Mn2+	maximal activity at 25 mM	Gallus gallus
Ni2+	can substitute partially for Mn2+	Gallus gallus
Ni2+	14% activity compared to Mn2+	Gallus gallus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	hen	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
oviduct	-	Gallus gallus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	assay method development	Gallus gallus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-pyridylamine	fluorescent synthetic substrate	Gallus gallus	UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-pyridylamine	-	?

Synonyms

Synonyms	Comment	Organism
UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI	-	Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Gallus gallus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	Hepes buffer	Gallus gallus

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Release 2023.1 (January 2023)