Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
strain YM4 (AB013109). To convert cellobiose into amylose: the cellobiose phosphorylase catalyzes phosphorolysis of cellobiose into G1-P and glucose, then alpha-glucan phosphorylase catalyses the polymerization of alpha-1,4-glucan, using G1-P as a carbohydrate donor and maltotetraose as the initial carbohydrate acceptor. Thus, glucosyl moieties of cellobiose molecules are successively transferred to the non-reducing ends of glucan to elongate the chain. The optimal conditions are: thirty milligrams per milliliters (87.7 mM) cellobiose, 30 mM sodium phosphate (pH 7.0), 30 microg/ml cellobiose phosphorylase, 30 microg/ml alpha-glucan phosphorylase, and 75 microM maltotetraose are incubated at 45°C | - |
Acetivibrio thermocellus YM4 | - |
strain YM4 (AB013109). To convert cellobiose into amylose: the cellobiose phosphorylase catalyzes phosphorolysis of cellobiose into G1-P and glucose, then alpha-glucan phosphorylase catalyses the polymerization of alpha-1,4-glucan, using G1-P as a carbohydrate donor and maltotetraose as the initial carbohydrate acceptor. Thus, glucosyl moieties of cellobiose molecules are successively transferred to the non-reducing ends of glucan to elongate the chain. The optimal conditions are: thirty milligrams per milliliters (87.7 mM) cellobiose, 30 mM sodium phosphate (pH 7.0), 30 microg/ml cellobiose phosphorylase, 30 microg/ml alpha-glucan phosphorylase, and 75 microM maltotetraose are incubated at 45°C | - |
Purification (Comment) | Organism |
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Ni-NTA agarose slurry, Superdex 200 column and Mono Q column | Acetivibrio thermocellus |