Application | Comment | Organism |
---|---|---|
synthesis | the cellobiose phosphorylases in the cell extract is used to synthesize radiolabeled cellodextrins with a degree of polymerization (DP=2-6) from non-radioactive glucose-1-phosphate and radioactive glucose. For cellobiose synthesis, the reaction is carried out at 60°C for 30 min with crude enzyme. After an impluse feed of radiolabeled cellobiose to a Clostridium thermocellum culture, the intracellular sugar levels are measured: the largest amount of radioactivity is cellobiose with lesser amounts of glucose, cellotriose, and cellotetraose, and an average degree of polymerization of intracellular cellodextrins is ca. 2 | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
strain ATCC 27405 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the cellobiose phosphorylase activites in the concentrated cell extract is 84 IU/ml. | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
Cbp | - |
Acetivibrio thermocellus |