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Literature summary for 2.4.1.20 extracted from

  • Zhang, Y.H.; Lynd, L.R.
    Biosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars (2006), Appl. Microbiol. Biotechnol., 70, 123-129.
    View publication on PubMed

Application

Application Comment Organism
synthesis the cellobiose phosphorylases in the cell extract is used to synthesize radiolabeled cellodextrins with a degree of polymerization (DP=2-6) from non-radioactive glucose-1-phosphate and radioactive glucose. For cellobiose synthesis, the reaction is carried out at 60°C for 30 min with crude enzyme. After an impluse feed of radiolabeled cellobiose to a Clostridium thermocellum culture, the intracellular sugar levels are measured: the largest amount of radioactivity is cellobiose with lesser amounts of glucose, cellotriose, and cellotetraose, and an average degree of polymerization of intracellular cellodextrins is ca. 2 Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
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strain ATCC 27405
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the cellobiose phosphorylase activites in the concentrated cell extract is 84 IU/ml. Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
Cbp
-
Acetivibrio thermocellus