Application | Comment | Organism |
---|---|---|
synthesis | ability of the phosphorylase to utilize alpha-D -glucose 1-fluoride as alternate glucosyl donor in place of alpha-D-glucose 1-phosphate for the synthesis of alpha-1,4-glucosides under thermodynamic control in close to 100% yield | Cellulomonas uda |
Cloned (Comment) | Organism |
---|---|
expression of a functional recombinant enzyme containing an N-terminal metal affinity fusion peptide in Escherichia coli. Catalytic properties of the natural enzyme are retained completely in the recombinant cellobiose phosphorylase | Cellulomonas uda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | substrate inhibition | Cellulomonas uda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
phosphate | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
1.2 | - |
phosphate | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
2.15 | - |
alpha-D-glucose 1-phosphate | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
2.26 | - |
D-glucose | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
2.9 | - |
cellobiose | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
3.28 | - |
alpha-D-glucose 1-phosphate | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
3.79 | - |
D-glucose | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
4.3 | - |
cellobiose | pH 6.6, 30°C, native enzyme | Cellulomonas uda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Cellulomonas uda |
92000 | - |
1 * 92000, SDS-PAGE | Cellulomonas uda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas uda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Cellulomonas uda |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cellulomonas uda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D -glucose 1-fluoride + D-glucose | - |
Cellulomonas uda | cellobiose + fluoride | - |
ir | |
alpha-D-glucose 1-phosphate + D-glucose | - |
Cellulomonas uda | cellobiose + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 92000, SDS-PAGE | Cellulomonas uda |
Synonyms | Comment | Organism |
---|---|---|
CuCbP | - |
Cellulomonas uda |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.4 | - |
phosphate | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
15.4 | - |
phosphate | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
27.5 | - |
D-glucose | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
27.5 | - |
D-glucose | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
29.1 | - |
cellobiose | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
29.1 | - |
cellobiose | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda | |
43.2 | - |
alpha-D-glucose 1-phosphate | pH 6.6, 30°C, native enzyme | Cellulomonas uda | |
43.2 | - |
alpha-D-glucose 1-phosphate | pH 6.6, 30°C, recombinant His6-tagged enzyme | Cellulomonas uda |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
202 | - |
D-glucose | pH 6.6, 30°C, substrate inhibition, native enzyme | Cellulomonas uda | |
255 | - |
D-glucose | pH 6.6, 30°C, substrate inhibition, recombinant His6-tagged enzyme | Cellulomonas uda |