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Literature summary for 2.4.1.20 extracted from

  • Kitaoka, M.; Sasaki, T.; Taniguchi, H.
    Synthetic reaction of Cellvibrio gilvus cellobiose phosphorylase (1992), J. Biochem., 112, 40-44.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-deoxy-D-glucose substrate inhibition Cellulomonas gilvus
D-glucosamine substrate inhibition Cellulomonas gilvus
D-glucose substrate inhibition, competitive with glucose-1-phosphate Cellulomonas gilvus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
D-glucose
-
Cellulomonas gilvus
13
-
D-glucosamine
-
Cellulomonas gilvus
24
-
6-deoxy-D-glucose
-
Cellulomonas gilvus
84
-
D-xylose
-
Cellulomonas gilvus
115
-
D-mannose
-
Cellulomonas gilvus
168
-
2-deoxy-D-glucose
-
Cellulomonas gilvus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Cellulomonas gilvus
-
alpha-D-glucose 1-phosphate + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Cellulomonas gilvus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose poor substrate Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose much better substrate than 2-deoxy-D-glucose Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-glucosamine poor substrate Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-mannose poor substrate Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-xylose poor substrate Cellulomonas gilvus ?
-
?
cellobiose + phosphate
-
Cellulomonas gilvus alpha-D-glucose 1-phosphate + D-glucose
-
?
cellobiose + phosphate beta-D-cellobiose is phosphorylyzed faster than alpha-anomer, ordered bi-bi mechanism Cellulomonas gilvus alpha-D-glucose 1-phosphate + D-glucose beta-D-glucose was shown to be the 4 times better acceptor compared to alpha-D-glucose in the reverse reaction ?