General Stability | Organism |
---|---|
storage of purified enzyme at -20°C without thiol reagents causes rapid loss of activity | Cellulomonas gilvus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-oxogluconate | - |
Cellulomonas gilvus | |
glucono-delta-lactone | very weak | Cellulomonas gilvus | |
N-ethylmaleimide | 56% inhibition at 0.5 mM | Cellulomonas gilvus | |
Nojirimycin | competitive with respect to cellobiose | Cellulomonas gilvus | |
p-chloromercuribenzoate | complete inhibition at 0.02 mM | Cellulomonas gilvus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
phosphate | - |
Cellulomonas gilvus | |
1.25 | - |
cellobiose | - |
Cellulomonas gilvus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cellulomonas gilvus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
SDS-PAGE | Cellulomonas gilvus |
280000 | - |
gel filtration | Cellulomonas gilvus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | Cellulomonas gilvus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas gilvus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cellulomonas gilvus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.4 | - |
purified enzyme | Cellulomonas gilvus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | - |
Cellulomonas gilvus | alpha-D-glucose 1-phosphate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 72000, gel filtration, SDS-PAGE | Cellulomonas gilvus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivated above | Cellulomonas gilvus |
60 | - |
complete inactivation within 10 min | Cellulomonas gilvus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Cellulomonas gilvus |