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Literature summary for 2.4.1.20 extracted from
- Purification and specificity of cellobiose phosphorylase from Clostridium thermocellum (1968), J. Biol. Chem., 243, 2899-2904.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | product inhibition | Acetivibrio thermocellus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
phosphate | - |
Acetivibrio thermocellus | |
| 7.3 | - |
cellobiose | - |
Acetivibrio thermocellus | |
| 9.2 | - |
6-deoxy-D-glucose | - |
Acetivibrio thermocellus | |
| 9.5 | - |
D-glucosamine | - |
Acetivibrio thermocellus | |
| 35 | - |
D-xylose | - |
Acetivibrio thermocellus | |
| 73 | - |
2-deoxy-D-glucose | - |
Acetivibrio thermocellus | |
| 85 | - |
D-mannose | - |
Acetivibrio thermocellus | |
| 160 | - |
L-fucose | - |
Acetivibrio thermocellus | |
| 240 | - |
D-arabinose | - |
Acetivibrio thermocellus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + phosphate | Acetivibrio thermocellus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.3 | - |
purified enzyme | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-altrose | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-arabinose | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-glucosamine | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-mannose | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-xylose | no activity with L-isomer | Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + L-fucose | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-D-glucose 1-phosphate + L-galactose | - |
Acetivibrio thermocellus | ? | - |
? | |
| cellobiose + phosphate | - |
Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
with D-xylose and 2-deoxyglucose as acceptor | Acetivibrio thermocellus |
| 7.5 | - |
with D-glucosamine as acceptor | Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | - |
Acetivibrio thermocellus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-glucose | product inhibition | Acetivibrio thermocellus | |
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