Cloned (Comment) | Organism |
---|---|
recombinant expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)-CodonPlus-RIL | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the extracellular enzyme is secreted, liberation from the spheroplast cells | Gluconobacter oxydans | - |
- |
intracellular | cell surface, the cell-associated form (DDint) appears after depletion of glucose from the medium | Gluconobacter oxydans | 5622 | - |
additional information | extracellular and cell-associated forms of the enzyme change their localization depending on the cell growth. Genomic southern blot, peptide mass fingerprinting and reaction product-pattern analyses reveal that the enzyme forms are structurally identical | Gluconobacter oxydans | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | A0A0M5MSC0 | - |
- |
Gluconobacter oxydans ATCC 11894 | A0A0M5MSC0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-CodonPlus-RIL by nickel affinity chromatography, dialysis, and ultrafiltration | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose | Gluconobacter oxydans | ? | - |
? | |
additional information | substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose | Gluconobacter oxydans ATCC 11894 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDase | - |
Gluconobacter oxydans |
dextran dextrinase | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
assay at | Gluconobacter oxydans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme synthesizes dextran from the starch hydrolysate, dextrin | Gluconobacter oxydans |