Cloned (Comment) | Organism |
---|---|
genes involved in GPI-anchor synthesis in Caenorhabditis elegans, overview. Isolation and analysis of piga-1-knockout allele tm2939 | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the RNAi phenotypes of each gene, including larval arrest, scrawny larvae, and germline defects, may result from N-/O-glycosylation inhibition as well as GPI-anchor inhibition | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | Caenorhabditis elegans | - |
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
gene piga-1 encoding the catalytic subunit of the phosphatidylinositol N-acetylglucosaminyltransferase complex | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
germ cell | - |
Caenorhabditis elegans | - |
gonad | - |
Caenorhabditis elegans | - |
additional information | the wild-type PIGA-1 protein is solely detected in the germline, and no EGFP-tagged PIGA-1 is detected in somatic cells | Caenorhabditis elegans | - |
muscle | - |
Caenorhabditis elegans | - |
neuron | - |
Caenorhabditis elegans | - |
oocyte | - |
Caenorhabditis elegans | - |
sperm | - |
Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | - |
Caenorhabditis elegans | UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycosylphosphatidylinositol N-acetylglucosaminyltransferase complex | - |
Caenorhabditis elegans |
GPI-GnT | - |
Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
malfunction | piga-1-knockout worms show 100% lethality, with decreased mitotic germline cells and abnormal eggshell formation. Cell-specific rescue of the null allele, expression of piga-1 in somatic gonads and/or in germline is sufficient for normal embryonic development and the maintenance of the germline mitotic cells. The RNAi phenotypes of each gene, including larval arrest, scrawny larvae, and germline defects, may result from N-/O-glycosylation inhibition as well as GPI-anchor inhibition | Caenorhabditis elegans |
physiological function | the phosphatidylinositol N-acetylglucosaminyltransferase complex catalyzes the first step of GPI-anchor synthesis, which is indispensable for the germline development of the nematode Caenorhabditis elegans. GPI-anchor synthesis is indispensable for the maintenance of mitotic germline cell number | Caenorhabditis elegans |