Literature summary for 2.4.1.198 extracted from

Li, H.; Zhou, H.; Luo, Y.; Ouyang, H.; Hu, H.; Jin, C.
Glycosylphosphatidylinositol (GPI) anchor is required in Aspergillus fumigatus for morphogenesis and virulence (2007), Mol. Microbiol., 64, 1014-1027.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha	Aspergillus fumigatus

Protein Variants

Protein Variants	Comment	Organism
additional information	afpig-a null mutant, strain CEA17 (pyrG-). This mutant shows a complete loss in glycosylphosphatidylinositol-N-acetylglucoaminyltransferase activity. None of the membrane bound glycosylphosphatidylinositol-anchored phospholipases, phosphatase, eCM33por Gl1p is found in the membrane preparation of the mutant. The mutant can grow at temperatures from 30°C to 50°C, but the growth rates are greatly inhibited. When the mutant is grown in the presence of various cell wall-disrupting agents, it is more sensitive to Calcofluor white, Congo-red, Nikkomycin Z and SDS, than the wild type. The mannoproteins and beta-glucans in mycelial cell wall of the mutant is 2.5fold and 2fold of the wild type or revertant respectively. The deletion of the afpig-a gene leads to earlier polarity, germ tube emergence and septation of mutant conidiospores in the early duplication cycles and to significant changes in developmental events and morphogenesis during conidiation. Two virulence factors, mycelial catalase Cat1 and Asp-haemolysin, are missing in the mutant, but the chitinase ChiB is found remarkably increased. After the inoculation of wild-type and mutant conidia into immunocompromised mice, early mortality is nearly identical among all groups. The remaining mice receiving the deletion mutant survived significantly longer.	Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	Q6Q2B4	afpig-a; Most common opportunistic fungal pathogen of human	-
Aspergillus fumigatus YJ-407	Q6Q2B4	afpig-a; Most common opportunistic fungal pathogen of human	-

Synonyms

Synonyms	Comment	Organism
afpig-a	-	Aspergillus fumigatus
afpig-a	gene is the homologue of GPI3 /SPT14 /pig-A gene (encode the catalytic subunit of glycosylphosphatidylinositol-N-acetylglucoaminyltransferase complex) in Aspergillus fumigatus	Aspergillus fumigatus
GPI-GnT	-	Aspergillus fumigatus
GPI-N-acetylglucosaminyltransferase complex	-	Aspergillus fumigatus

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Release 2023.1 (January 2023)