Literature summary for 2.4.1.198 extracted from

Murakami, Y.; Siripanyaphinyo, U.; Hong, Y.; Tashima, Y.; Maeda, Y.; Kinoshita, T.
The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component (2005), Mol. Biol. Cell, 16, 5236-5246.

Search for more literature for 2.4.1.198

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Daudi cells. Daudi cells have no PIG-Y transcritpt. PIG-Y cDNA restores the surface expression of CD59, DAF and CD48, but an empty vector does not. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase activity is also restored	Homo sapiens
expressed in HeLa cells	Homo sapiens
in chinese hamster ovary cells	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q3MUY2	cDNA that encode a 71-amino acid protein, which is termed PIG-Y (for phosphatidylinositlglycan-class Y). Mouse and rice homologues of PIG-Y, exhibit 79% and 25% amino acid identity with human PIG-Y, respectively. The hydropathy profile of Saccharomyces cerevisiae´s Eri1p is quite similar to PIG-Y and the amino acid identity is 22%. N-terminus and C-terminus face the cytoplasmic side.; from the human T lymphoma KT-3 cDNA library	-

Purification (Commentary)

Purification (Comment)	Organism
affinity purified	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	overexpression of active froms of the Ras family of proteins does not affect glycosylphosphatidylinositol-N-acetylglucosaminyltransferase activity	Homo sapiens

Subunits

Subunits	Comment	Organism
More	the six other components of glycosylphosphatidylinositol-N-acetylglucosaminyltransferase can form a complex without PIG-Y, but this complex does not have any detectable glycosylphosphatidylinositol-N-acetylglucosaminyltransferase activity. In PIG-A-deficient JY5 cells, PIG-Y is not coprecipitated with any other components. PIG-Y associates directly with PIG-A and indirectly with other components through the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase complex. Additionally, there is no detectable association between PIG-Y and any of Ras proteins	Homo sapiens

Synonyms

Synonyms	Comment	Organism
GPI-GnT	-	Homo sapiens
GPI-N-acetylglucosaminyltransferase	-	Homo sapiens

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Release 2023.1 (January 2023)