Any feedback?
Literature summary for 2.4.1.195 extracted from
- Purification and properties of a UDP glucose:thiohydroximate glucosyltransferase from higher plants (1971), Phytochemistry, 10, 2279-2288.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.1 mM, activation | Tropaeolum majus |  |
| 2,2'-dipyridyl | 1.0 mM, activation | Tropaeolum majus | |
| 2-mercaptoethanol | requirement | Tropaeolum majus | |
| 2-mercaptoethanol | activity dissappears in absence | Tropaeolum majus | |
| Cysteine hydrochloride | 1.0 mM, activation | Tropaeolum majus | |
| dithiothreitol | 1.0 mM, activation | Tropaeolum majus | |
| EDTA | 0.01 mM, activation | Tropaeolum majus | |
| glutathione | 1.0 mM, activation | Tropaeolum majus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Tropaeolum majus | |
| additional information | no inhibition by KCN or iodoacetic acid, only moderately inhibited by N-ethylmaleimide | Tropaeolum majus | |
| p-chloromercuribenzoate | - |
Tropaeolum majus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.05 | - |
4-methylthiobutyrothiohydroximate | pH 7.4, 30°C | Tropaeolum majus | |
| 1.47 | - |
UDPglucose | pH 7.4, 30°C, 4-methylthiobutyrothiohydroximate | Tropaeolum majus | |
| 1.54 | - |
UDPglucose | pH 7.4, 30°C, phenylacetothiohydroximate | Tropaeolum majus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Tropaeolum majus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + phenylacetothiohydroximate | Sinapis alba | - |
UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | Nasturtium officinale | - |
UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | Armoracia rusticana | - |
UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | Tropaeolum majus | integral step in the biosynthesis of benzylglucosinolate | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | Nasturtium officinale R.Br. | - |
UDP + desulfoglucotropeolin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Armoracia rusticana | - |
- |
- |
| Nasturtium officinale | - |
R.Br. | - |
| Nasturtium officinale R.Br. | - |
R.Br. | - |
| Sinapis alba | - |
- |
- |
| Tropaeolum majus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tropaeolum majus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Sinapis alba | - |
| leaf | - |
Tropaeolum majus | - |
| leaf | - |
Nasturtium officinale | - |
| leaf | - |
Armoracia rusticana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Tropaeolum majus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, freeze-dried protein, specific activity remains unchanged for more than 3 months | Tropaeolum majus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | acetothiohydroximate, phenylacetohydroximic acid, ethanol, 2-mercaptoethanol and cysteine hydrochloride are no substrates | Tropaeolum majus | ? | - |
? | |
| UDP-glucose + 4-methylthiobutyrothiohydroximate | relative reaction rate 77% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-4-methylthiobutyrothiohydroximate | - |
? | |
| UDP-glucose + benzothiohydroximate | relative reaction rate 10% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-benzothiohydroximate | - |
? | |
| UDP-glucose + isobutyrothiohydroximate | relative reaction rate 41% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-isobutyrothiohydroximate | - |
? | |
| UDPglucose + butyrothiohydroximate | relative reaction rate 70% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-butyrothiohydroximate | - |
? | |
| UDPglucose + phenylacetothiohydroximate | - |
Sinapis alba | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | - |
Tropaeolum majus | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | - |
Nasturtium officinale | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | - |
Armoracia rusticana | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | integral step in the biosynthesis of benzylglucosinolate | Tropaeolum majus | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | - |
Nasturtium officinale R.Br. | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + propiothiohydroximate | relative reaction rate 50% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-propiothiohydroximate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | greater activity in phosphate and Tris-HCl buffers than in Tris-maleate | Tropaeolum majus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
