Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chitosan | the beta-CGTase and gamma-CGTase activities increase as the chitosan concentrations increases to 2% (w/v) | Alkalihalobacillus pseudalcaliphilus | |
ethylene glycol | with increasing concentrations of ethylene glycol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control | Alkalihalobacillus pseudalcaliphilus | |
gelatin | with increasing gelatin concentrations, both the beta-CGTase and gamma-CGTase activities show bell-shaped curves, with the highest increases of activities of 48.9% and 38.6%, respectively, at 1% (w/v) gelatin | Alkalihalobacillus pseudalcaliphilus | |
glycerol | with increasing concentrations of glycerol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control | Alkalihalobacillus pseudalcaliphilus | |
mycose | the beta-CGTase and gamma-CGTase activities increase as the mycose concentrations increases to 2% (w/v) | Alkalihalobacillus pseudalcaliphilus | |
PEG400 | the beta-CGTase and gamma-CGTase activities increase by 50.4% and 55.3% compared with that of control at the condition of 10% (w/v) PEG400 | Alkalihalobacillus pseudalcaliphilus | |
Sodium acetate | the highest increase in beta-CGTase activity is 46.9% at 1 mM sodium acetate which is 5.8fold higher than that of the control. The highest increase in gamma-CGTase activity is 39.1% at 2 mM sodium acetate which is 5.0fold higher than that of the control | Alkalihalobacillus pseudalcaliphilus |
Cloned (Comment) | Organism |
---|---|
expressed in Komagataella phaffii | Alkalihalobacillus pseudalcaliphilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the highest increase in beta-CGTase activity is 54.3% at 1 mM CaCl2 which is 5.6fold higher than that of the control. The highest increase in gamma-CGTase activity is 55.5% 1 mM CaCl2 which is 7.2fold higher than that of the control | Alkalihalobacillus pseudalcaliphilus | |
Mg2+ | the highest increase in beta-CGTase activity is 46.4% at 2 mM MgSO4 which is 4.8fold higher than that of the control. The highest increase in gamma-CGTase activity is 45.9% at 3 mM MgSO4 which is 5.9fold higher than that of the control | Alkalihalobacillus pseudalcaliphilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus pseudalcaliphilus | - |
- |
- |
Alkalihalobacillus pseudalcaliphilus 8SB | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
potato starch + glycosyl acceptor | - |
Alkalihalobacillus pseudalcaliphilus | beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
potato starch + glycosyl acceptor | - |
Alkalihalobacillus pseudalcaliphilus 8SB | beta-cyclodextrin + gamma-cyclodextrin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-CGTase | - |
Alkalihalobacillus pseudalcaliphilus |
CGTase | - |
Alkalihalobacillus pseudalcaliphilus |
cyclodextrin glucanotransferase | - |
Alkalihalobacillus pseudalcaliphilus |
gamma-CGTase | - |
Alkalihalobacillus pseudalcaliphilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Alkalihalobacillus pseudalcaliphilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | a chemical mixture (25% (v/v) glycerol, 10% (w/v) polyethylene glycol 400, and 0.5 mM CaCl2) is used as stabilizer to enhance the thermostability of the enzyme. The beta- and gamma-cyclodextrin glucanotransferase activities increase by 7.6- and 9.4-fold, respectively, compared with controls. The optimum temperature of enzyme activity increases from 50 to 60°C after the stabilizer application | Alkalihalobacillus pseudalcaliphilus |