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Literature summary for 2.4.1.19 extracted from
- Current studies on the enzymatic preparation 2-O-alpha-D-glucopyranosyl-L-ascorbic acid with cyclodextrin glycosyltransferase (2019), Crit. Rev. Biotechnol., 39, 249-257 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.25 | - |
beta-cyclodextrin | at pH 5.0 and 35°C | Anaerobranca gottschalkii |  |
| 1.31 | - |
beta-cyclodextrin | at pH 5.0 and 35°C | Niallia circulans | |
| 1.51 | - |
beta-cyclodextrin | at pH 5.5 and 37°C | Paenibacillus macerans | |
| 18.3 | - |
L-ascorbic acid | at pH 5.0 and 35°C | Niallia circulans | |
| 19.6 | - |
L-ascorbic acid | at pH 5.0 and 35°C | Anaerobranca gottschalkii | |
| 25.5 | - |
L-ascorbic acid | at pH 5.5 and 37°C | Paenibacillus macerans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalobacillus alcalophilus | - |
- |
- |
| Alkalihalobacillus alcalophilus 7-12 | - |
- |
- |
| Anaerobranca gottschalkii | - |
- |
- |
| Bacillus sp. SK 13.002 | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Niallia circulans | - |
- |
- |
| Niallia circulans 251 | - |
- |
- |
| Paenibacillus macerans | - |
- |
- |
| Paenibacillus sp. | - |
- |
- |
| Paenibacillus sp. JB-13 | - |
- |
- |
| Paenibacillus sp. JK-12 | - |
- |
- |
| Thermoanaerobacter sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| alpha-cyclodextrin + L-ascorbic acid | highest activity | Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| alpha-cyclodextrin + L-ascorbic acid | 30% conversion rate | Thermoanaerobacter sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| alpha-cyclodextrin + L-ascorbic acid | highest activity, 40% conversion rate | Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| amylose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | 1% conversion rate | Alkalihalobacillus alcalophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | 30% conversion rate | Anaerobranca gottschalkii | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | 37% conversion rate | Niallia circulans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | 9% conversion rate | Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | 1% conversion rate | Alkalihalobacillus alcalophilus 7-12 | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| beta-cyclodextrin + L-ascorbic acid | 37% conversion rate | Niallia circulans 251 | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| corn starch + L-ascorbic acid | - |
Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| corn starch + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| dextrin + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| dextrin + L-ascorbic acid | - |
Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| dextrin + L-ascorbic acid | highest activity, 17% conversion rate | Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| gamma-cyclodextrin + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| gamma-cyclodextrin + L-ascorbic acid | - |
Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| gamma-cyclodextrin + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltoheptaose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltoheptaose + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltohexaose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid + ? | - |
? | |
| maltopentaose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltose + L-ascorbic acid | - |
Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltose + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltose + L-ascorbic acid | 20% conversion rate | Bacillus sp. SK 13.002 | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltotetraose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltotriose + L-ascorbic acid | - |
Geobacillus stearothermophilus | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| maltotriose + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| additional information | no activity with D-glucose as glycosyl donor | Geobacillus stearothermophilus | ? | - |
- |
|
| additional information | no activity with D-glucose as glycosyl donor | Paenibacillus macerans | ? | - |
- |
|
| additional information | no activity with D-glucose as glycosyl donor | Paenibacillus sp. | ? | - |
- |
|
| potato starch + L-ascorbic acid | - |
Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| potato starch + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| rice starch + L-ascorbic acid | - |
Paenibacillus macerans | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| rice starch + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| soluble starch + L-ascorbic acid | - |
Paenibacillus sp. | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| soluble starch + L-ascorbic acid | - |
Paenibacillus sp. JK-12 | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
| soluble starch + L-ascorbic acid | - |
Paenibacillus sp. JB-13 | 2-O-alpha-D-glucopyranosyl-L-ascorbic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CGTase | - |
Geobacillus stearothermophilus |
| CGTase | - |
Niallia circulans |
| CGTase | - |
Paenibacillus macerans |
| CGTase | - |
Alkalihalobacillus alcalophilus |
| CGTase | - |
Thermoanaerobacter sp. |
| CGTase | - |
Paenibacillus sp. |
| CGTase | - |
Anaerobranca gottschalkii |
| CGTase | - |
Paenibacillus sp. JK-12 |
| CGTase | - |
Paenibacillus sp. JB-13 |
| CGTase | - |
Bacillus sp. SK 13.002 |
| cyclodextrin glucosyltransferase | - |
Geobacillus stearothermophilus |
| cyclodextrin glucosyltransferase | - |
Niallia circulans |
| cyclodextrin glucosyltransferase | - |
Paenibacillus macerans |
| cyclodextrin glucosyltransferase | - |
Alkalihalobacillus alcalophilus |
| cyclodextrin glucosyltransferase | - |
Thermoanaerobacter sp. |
| cyclodextrin glucosyltransferase | - |
Paenibacillus sp. |
| cyclodextrin glucosyltransferase | - |
Anaerobranca gottschalkii |
| cyclodextrin glucosyltransferase | - |
Paenibacillus sp. JK-12 |
| cyclodextrin glucosyltransferase | - |
Paenibacillus sp. JB-13 |
| cyclodextrin glucosyltransferase | - |
Bacillus sp. SK 13.002 |
| cyclomaltodextrin glycanotransferase | - |
Geobacillus stearothermophilus |
| cyclomaltodextrin glycanotransferase | - |
Niallia circulans |
| cyclomaltodextrin glycanotransferase | - |
Paenibacillus macerans |
| cyclomaltodextrin glycanotransferase | - |
Alkalihalobacillus alcalophilus |
| cyclomaltodextrin glycanotransferase | - |
Thermoanaerobacter sp. |
| cyclomaltodextrin glycanotransferase | - |
Paenibacillus sp. |
| cyclomaltodextrin glycanotransferase | - |
Anaerobranca gottschalkii |
| cyclomaltodextrin glycanotransferase | - |
Paenibacillus sp. JK-12 |
| cyclomaltodextrin glycanotransferase | - |
Paenibacillus sp. JB-13 |
| cyclomaltodextrin glycanotransferase | - |
Bacillus sp. SK 13.002 |
| cyclomaltodextrin glycosyltransferase | - |
Geobacillus stearothermophilus |
| cyclomaltodextrin glycosyltransferase | - |
Niallia circulans |
| cyclomaltodextrin glycosyltransferase | - |
Paenibacillus macerans |
| cyclomaltodextrin glycosyltransferase | - |
Alkalihalobacillus alcalophilus |
| cyclomaltodextrin glycosyltransferase | - |
Thermoanaerobacter sp. |
| cyclomaltodextrin glycosyltransferase | - |
Paenibacillus sp. |
| cyclomaltodextrin glycosyltransferase | - |
Anaerobranca gottschalkii |
| cyclomaltodextrin glycosyltransferase | - |
Paenibacillus sp. JK-12 |
| cyclomaltodextrin glycosyltransferase | - |
Paenibacillus sp. JB-13 |
| cyclomaltodextrin glycosyltransferase | - |
Bacillus sp. SK 13.002 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Niallia circulans |
| 35 | - |
- |
Anaerobranca gottschalkii |
| 37 | - |
- |
Paenibacillus macerans |
| 37 | - |
- |
Bacillus sp. SK 13.002 |
| 45 | - |
- |
Paenibacillus sp. |
| 50 | - |
- |
Alkalihalobacillus alcalophilus |
| 50 | - |
- |
Thermoanaerobacter sp. |
| 55 | - |
- |
Paenibacillus sp. JK-12 |
| 55 | - |
- |
Paenibacillus sp. JB-13 |
| 60 | - |
- |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Thermoanaerobacter sp. |
| 5 | - |
- |
Niallia circulans |
| 5 | - |
- |
Anaerobranca gottschalkii |
| 5.5 | - |
- |
Geobacillus stearothermophilus |
| 5.5 | - |
- |
Paenibacillus macerans |
| 5.5 | - |
- |
Paenibacillus sp. JK-12 |
| 6 | - |
- |
Paenibacillus sp. |
| 6 | - |
- |
Paenibacillus sp. JB-13 |
| 7 | - |
- |
Alkalihalobacillus alcalophilus |
| 8 | - |
- |
Bacillus sp. SK 13.002 |
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