Cloned (Comment) | Organism |
---|---|
mutant enzymes I631T, I641T and K647E are expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
I631T | the specific activities of the mutant is slightly higher than that of the wild type enzyme. The mutation has negligible effects on either the temperature and pH optima of the enzyme or its temperature and pH stability | Geobacillus stearothermophilus |
I641T | the specific activities of the mutant is slightly higher than that of the wild type enzyme. The mutation has negligible effects on either the temperature and pH optima of the enzyme or its temperature and pH stability | Geobacillus stearothermophilus |
K647E | the specific activities of the mutant is slightly higher than that of the wild type enzyme. The mutation has negligible effects on either the temperature and pH optima of the enzyme or its temperature and pH stability | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P31797 | - |
- |
Geobacillus stearothermophilus NO2 | P31797 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
450 | - |
wild type enzyme, at pH 6.0 and 50°C | Geobacillus stearothermophilus |
456 | - |
mutant enzyme I641T, at pH 6.0 and 50°C | Geobacillus stearothermophilus |
463 | - |
mutant enzyme I631T, at pH 6.0 and 50°C | Geobacillus stearothermophilus |
487 | - |
mutant enzyme K647E, at pH 6.0 and 50°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-maltoheptaoside-4-6-O-ethylidene + maltose | - |
Geobacillus stearothermophilus | ? | - |
? | |
4-nitrophenyl alpha-D-maltoheptaoside-4-6-O-ethylidene + maltose | - |
Geobacillus stearothermophilus NO2 | ? | - |
? | |
L-ascorbic acid + maltodextrin | - |
Geobacillus stearothermophilus | L-ascorbic acid-2-O-alpha-D-glucoside + ? | - |
? | |
L-ascorbic acid + maltodextrin | - |
Geobacillus stearothermophilus NO2 | L-ascorbic acid-2-O-alpha-D-glucoside + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
CGT | - |
Geobacillus stearothermophilus |
CGTase | - |
Geobacillus stearothermophilus |
cyclodextrin glucosyltransferase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme remains stable (more than 90% activity) for 120 min at 30°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | more than 70% activity between pH 5.0 and 7.0 | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the enzyme remains stable for 120 min at pH 5.0-8.0 | Geobacillus stearothermophilus |