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Literature summary for 2.4.1.19 extracted from
- A novel cyclodextrin glucanotransferase from alkaliphilic Bacillus pseudalcaliphilus 20RF: purification and properties (2011), Process Biochem., 46, 116-122.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme achieves 47% conversion of an insoluble raw commercial corn starch into cyclodextrins with production of only beta- and gamma-cyclodextrins, in a ratio of 80%:20% in alkaline pH 9.0 | Alkalihalobacillus pseudalcaliphilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 6 mM, 62% residual activity | Alkalihalobacillus pseudalcaliphilus |  |
| Fe2+ | 6 mM, 80% residual activity | Alkalihalobacillus pseudalcaliphilus | |
| Mg2+ | 6 mM, 57% residual activity | Alkalihalobacillus pseudalcaliphilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Alkalihalobacillus pseudalcaliphilus |
| 70000 | - |
1 * 70000, SDS-PAGE | Alkalihalobacillus pseudalcaliphilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalobacillus pseudalcaliphilus | - |
- |
- |
| Alkalihalobacillus pseudalcaliphilus 20RF | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alkalihalobacillus pseudalcaliphilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6638 | - |
pH 6.0, 60°C | Alkalihalobacillus pseudalcaliphilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raw starch | - |
Alkalihalobacillus pseudalcaliphilus | beta-cyclodextrin + gamma-cyclodextrin | sole products, beta- and gamma-cyclodextrin in a ratio of 80%:20% | ? | |
| raw starch | - |
Alkalihalobacillus pseudalcaliphilus 20RF | beta-cyclodextrin + gamma-cyclodextrin | sole products, beta- and gamma-cyclodextrin in a ratio of 80%:20% | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Alkalihalobacillus pseudalcaliphilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Alkalihalobacillus pseudalcaliphilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | - |
Alkalihalobacillus pseudalcaliphilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme is more heat resistant after its pretreatment in pH 9.0 at high temperatures 65-70°C, than at pH 6.0 under the same reaction conditions | Alkalihalobacillus pseudalcaliphilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
and 9.0 | Alkalihalobacillus pseudalcaliphilus |
| 9 | - |
and 6.0 | Alkalihalobacillus pseudalcaliphilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Alkalihalobacillus pseudalcaliphilus |
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Release 2023.1 (January 2023)
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