Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 105% of initial activity | Paenibacillus macerans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paenibacillus macerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, 5% of initial activity | Paenibacillus macerans | |
Ni2+ | 1 mM, 58% of initial activity | Paenibacillus macerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data fit to Hill equation and lead to coefficients of 1.485 and 1.257 for the recombinant and the native enzyme, respectively | Paenibacillus macerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 0.5 mM, 125% of initial activity | Paenibacillus macerans | |
Ca2+ | 0.5 mM, 116% of initial activity | Paenibacillus macerans | |
Zn2+ | 0.5 mM, 118% of initial activity | Paenibacillus macerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
1 * 72000, SDS-PAGE | Paenibacillus macerans |
75000 | - |
gel filtration | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
- |
- |
Paenibacillus macerans JBF05-01 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch | - |
Paenibacillus macerans | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | at the initial stage of the reaction, alpha-cyclodextrin is the main product. Subsequently, the proportion of beta-cyclodextrin increases and becomes the main product after prolonged incubation. After 10 h or 40 h of incubation, the conversion rates of starch into cyclodextrins are 36.8% or 42.3%, respectively | ? | |
soluble starch | - |
Paenibacillus macerans JBF05-01 | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | at the initial stage of the reaction, alpha-cyclodextrin is the main product. Subsequently, the proportion of beta-cyclodextrin increases and becomes the main product after prolonged incubation. After 10 h or 40 h of incubation, the conversion rates of starch into cyclodextrins are 36.8% or 42.3%, respectively | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE | Paenibacillus macerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Paenibacillus macerans |
50 | - |
native enzyme | Paenibacillus macerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | more than 95% of maximum activity, recombinant enzyme | Paenibacillus macerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life about 8 h | Paenibacillus macerans |
45 | - |
half-life about 1.25 h | Paenibacillus macerans |
50 | - |
half-life about 0.5 h | Paenibacillus macerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzymes are more active in glycine/NaOH buffer compared with Na2HPO4/NaH2PO4 buffer at the same pH | Paenibacillus macerans |
5.5 | - |
- |
Paenibacillus macerans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9.5 | loss of cyclization activity above and below | Paenibacillus macerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Paenibacillus macerans |