Literature summary for 2.4.1.19 extracted from

Tanaka, M.; Muto, N.; Yamamoto, I.
Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic acid 2-O-alpha-glucoside formation (1991), Biochim. Biophys. Acta, 1078, 127-132.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
16	-	alpha-cyclodextrin	-	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
alpha-cyclodextrin + ascorbic acid	-	Geobacillus stearothermophilus	L-ascorbic acid-2-O-alpha-D-glucoside + L-ascorbic acid-2-O-alpha-D-oligoglucoside	-	r
alpha-cyclodextrin + isoascorbic acid	-	Geobacillus stearothermophilus	L-isoascorbic acid-2-O-alpha-D-glucoside + L-isoascorbic acid-2-O-alpha-D-oligoglucoside	-	r
amylose + glycosyl acceptor	-	Geobacillus stearothermophilus	?	-	?
beta-cyclodextrin + glycosyl acceptor	-	Geobacillus stearothermophilus	alpha-cyclodextrin + maltooligosaccharide	-	r
dextrin + glycosyl acceptor	-	Geobacillus stearothermophilus	cyclodextrins	-	r
gamma-cyclodextrin + glycosyl acceptor	-	Geobacillus stearothermophilus	maltooligosaccharide	-	r
glycogen + acceptor	-	Geobacillus stearothermophilus	beta-cyclodextrin	-	?
L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + glycosyl acceptor	-	Geobacillus stearothermophilus	L-ascorbic acid alpha-D-glucoside + D-glucosyl-[glycosyl acceptor]	-	r
L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + H2O	-	Geobacillus stearothermophilus	L-ascorbic acid-2-O-alpha-D-glucoside + D-glucose	-	r
L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + glycosyl acceptor	-	Geobacillus stearothermophilus	L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + D-glucosyl-[glycosyl acceptor]	-	r
L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + H2O	-	Geobacillus stearothermophilus	L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + D-glucose	-	r
L-ascorbic acid-(2-O-alpha-D-glucosyl)4 + glycosyl acceptor	-	Geobacillus stearothermophilus	L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + glucosyl-[glycosyl acceptor]	-	r
L-ascorbic acid-(2-O-alpha-D-glucosyl)4 + H2O	-	Geobacillus stearothermophilus	L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + D-glucose	-	r
L-ascorbic acid-2-O-alpha-D-glucoside + H2O	-	Geobacillus stearothermophilus	L-ascorbic acid + D-glucose	-	?
maltohexaose + L-ascorbic acid	-	Geobacillus stearothermophilus	L-ascorbic acid-(2-O-alpha-D-glucosyl)6	-	r
maltooligosaccharides + glycosyl acceptor	-	Geobacillus stearothermophilus	cyclodextrins	-	r
maltose + acceptor	-	Geobacillus stearothermophilus	?	-	r
maltose + ascorbic acid	-	Geobacillus stearothermophilus	L-ascorbic acid-2-O-alpha-D-glucoside + D-glucose	-	r
maltose + glycosyl acceptor	-	Geobacillus stearothermophilus	beta-cyclodextrin	-	r
maltotetraose + glycosyl acceptor	-	Geobacillus stearothermophilus	beta-cyclodextrin	-	r
maltotriose + glycosyl acceptor	-	Geobacillus stearothermophilus	beta-cyclodextrin	-	r
additional information	disaccharides are not substrates, except maltose	Geobacillus stearothermophilus	?	-	?
additional information	isomaltose, sucrose, melibiose, phenyl-alpha-D-glucoside, cellobiose and lactose are not acceptors, L-ascorbic acid-2-O-phosphate and, L-ascorbic acid-2-O-sulfate are not substrates	Geobacillus stearothermophilus	?	-	?
soluble starch + glycosyl acceptor	-	Geobacillus stearothermophilus	cyclodextrins	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	optimum for L-ascorbic acid-2-O-alpha-D-glucoside formation	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	optimum pH for L-ascorbic acid-2-O-alpha-D-glucoside formation	Geobacillus stearothermophilus