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Literature summary for 2.4.1.19 extracted from

  • Akimaru, K.; Yagi, T.; Yamamoto, S.
    Purification and properties of Bacillus coagulans cyclomaltodextrin glucanotransferase (1991), J. Ferment. Bioeng., 71, 322-328.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
-
Geobacillus stearothermophilus
cloned and overexpressed in Bacillus subtilis Paenibacillus macerans

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Geobacillus stearothermophilus
-
Paenibacillus macerans

General Stability

General Stability Organism
not inactivated by 2 M guanidium chloride Weizmannia coagulans

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Weizmannia coagulans
Al3+
-
Weizmannia coagulans
chromate
-
Weizmannia coagulans
Cr3+
-
Weizmannia coagulans
Cu2+
-
Bacillus sp. (in: Bacteria)
Cu2+
-
Weizmannia coagulans
Hg2+
-
Weizmannia coagulans
HgSO4
-
Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
beta-cyclodextrin cyclodextrin opening Paenibacillus macerans
0.25
-
gamma-cyclodextrin
-
Weizmannia coagulans
0.25
-
gamma-cyclodextrin cyclodextrin opening Paenibacillus macerans
0.51
-
beta-cyclodextrin cyclodextrin opening Weizmannia coagulans
0.59
-
alpha-cyclodextrin coupling Weizmannia coagulans
0.73
-
alpha-cyclodextrin cyclodextrin opening Weizmannia coagulans
0.88
-
gamma-cyclodextrin cyclodextrin opening Weizmannia coagulans
1.26
-
alpha-cyclodextrin coupling Paenibacillus macerans
2.32
-
alpha-cyclodextrin cyclodextrin opening Paenibacillus macerans

Metals/Ions

Metals/Ions Comment Organism Structure
BaCl2 relative activity 102.4% Weizmannia coagulans
Ca2+
-
Geobacillus stearothermophilus
Ca2+
-
Bacillus sp. (in: Bacteria)
Ca2+
-
Niallia circulans
Ca2+ contains 2 mol Ca2+ per mol of enzyme, increases heat stability, denaturation temperature shifted from 70°C to 88°C Weizmannia coagulans
Ca2+ contains 1 mol Ca2+ per mol of enzyme Paenibacillus macerans
CaCl2 relative activity 106.7% Weizmannia coagulans
FeSO4 relative activity 107.6% Weizmannia coagulans
Li2SO4 relative activity 106% Weizmannia coagulans
MgSO4 relative activity 103.2% Weizmannia coagulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
gel filtration Superose 6 Weizmannia coagulans
34000
-
gel filtration BioGel P-100 Weizmannia coagulans
35000
-
-
Bacillus sp. (in: Bacteria)
35000
-
-
Bacillus ohbensis
35000
-
gel filtration Superose 6 Paenibacillus macerans
38000
-
-
Bacillus sp. (in: Bacteria)
38000
-
gel filtration BioGel P-100 Paenibacillus macerans
64000
-
-
Bacillus subtilis
65000
-
-
Paenibacillus macerans
65000
-
sedimentation equilibrium, SDS-PAGE Weizmannia coagulans
65500
-
amino acid composition Weizmannia coagulans
66000
-
-
Priestia megaterium
68000
-
-
Geobacillus stearothermophilus
68000
-
-
Bacillus sp. (in: Bacteria)
68000
-
sedimentation equilibrium, amino acid composition Paenibacillus macerans
85000 88000
-
Niallia circulans
88000
-
-
Niallia circulans
200000
-
-
Niallia circulans

Organic Solvent Stability

Organic Solvent Comment Organism
sodium dodecyl sulfate not inactivated by 0.2% Weizmannia coagulans
urea not inactivated by 8 M Weizmannia coagulans

Organism

Organism UniProt Comment Textmining
Bacillus ohbensis
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus subtilis
-
no.313
-
Bacillus subtilis 313
-
no.313
-
Geobacillus stearothermophilus
-
TC-60
-
Geobacillus stearothermophilus TC-60
-
TC-60
-
Klebsiella oxytoca
-
formerly Klebsiella pneumoniae M5a1
-
Klebsiella pneumoniae
-
-
-
Niallia circulans
-
B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
Paenibacillus macerans
-
formerly Aeromonas macerans
-
Priestia megaterium
-
-
-
Weizmannia coagulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Weizmannia coagulans
-
Bacillus sp. (in: Bacteria)
-
Paenibacillus macerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
gamma-cyclodextrin, opening Paenibacillus macerans
0.02
-
beta-cyclodextrin, opening Paenibacillus macerans
1.24
-
gamma-cyclodextrin, opening Paenibacillus macerans
1.42
-
alpha-cyclodextrin, opening Paenibacillus macerans
1.7
-
beta-cyclodextrin, opening Weizmannia coagulans
2.4
-
maltotriose, disproportionation Paenibacillus macerans
14.83
-
alpha-cyclodextrin, opening Weizmannia coagulans
15.5
-
maltotriose, disproportionation Weizmannia coagulans
37.4
-
alpha-cyclodextrin, coupling Paenibacillus macerans
203.1
-
alpha-cyclodextrin, coupling Weizmannia coagulans
883
-
starch, cyclodextrin synthesis Weizmannia coagulans
904
-
-
Weizmannia coagulans
1027
-
-
Paenibacillus macerans

Storage Stability

Storage Stability Organism
-20°C, 0.05 M Tris-HCl, pH 7.0 or sodium acetate buffer, pH 6.5 for at least 6 months without loss of activity Weizmannia coagulans
4°C, pH 5.0-10.0, does not lose activity for 1 month Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus alpha-cyclodextrin + maltooligosaccharide
-
r
beta-cyclodextrin + glycosyl acceptor
-
Weizmannia coagulans alpha-cyclodextrin + maltooligosaccharide
-
r
beta-cyclodextrin + glycosyl acceptor
-
Paenibacillus macerans alpha-cyclodextrin + maltooligosaccharide
-
r
beta-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus TC-60 alpha-cyclodextrin + maltooligosaccharide
-
r
maltotriose + maltotetraose
-
Weizmannia coagulans maltopentaose
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin strain ATCC 21783, pH 4.5-4.7, producing ratio 23.5: 1.0: 1.0, pH 7.0 0.2: 6.0: 1.0 r
soluble starch + glycosyl acceptor
-
Priestia megaterium alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin product ratio 2.0:5.0:1.0 r
soluble starch + glycosyl acceptor
-
Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin product ratio 2.0:5.0:1.0 r
soluble starch + glycosyl acceptor
-
Bacillus subtilis alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin producing ratio 0.0: 0.0: 1.0 r
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin producing ratio 5.5: 8.0: 1.0 r
soluble starch + glycosyl acceptor
-
Bacillus ohbensis alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin producing ratio 0.0: 4.0: 1.0 r
soluble starch + glycosyl acceptor potato starch, sweet potato starch, rice starch, corn starch, wheat starch Paenibacillus macerans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin producing ratio 5.0: 2.0: 1.0 r
soluble starch + glycosyl acceptor potato starch, sweet potato starch, rice starch, corn starch, wheat starch Weizmannia coagulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin potato starch, 20 h at 50°C, ratio 8.1: 8.9: 1.0, various conditions, product proportions 1.0: 1.0: 0.3 r
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus TC-60 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin producing ratio 5.5: 8.0: 1.0 r
soluble starch + glycosyl acceptor
-
Bacillus subtilis 313 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin producing ratio 0.0: 0.0: 1.0 r
soluble starch + glycosyl acceptor
-
Bacillus subtilis gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Paenibacillus macerans gamma-cyclodextrin
-
?
soluble starch + glycosyl acceptor
-
Bacillus subtilis 313 gamma-cyclodextrin
-
r
soluble starch + sucrose
-
Weizmannia coagulans maltosylfructose
-
r

Subunits

Subunits Comment Organism
monomer
-
Bacillus sp. (in: Bacteria)
monomer
-
Niallia circulans
monomer
-
Paenibacillus macerans
monomer 1 * 65000, SDS-PAGE Weizmannia coagulans

Synonyms

Synonyms Comment Organism
C-CGTase
-
Weizmannia coagulans
M-CGTase
-
Paenibacillus macerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus sp. (in: Bacteria)
45
-
-
Niallia circulans
50
-
-
Bacillus sp. (in: Bacteria)
50
-
-
Niallia circulans
55
-
-
Geobacillus stearothermophilus
55
-
-
Priestia megaterium
55
-
-
Weizmannia coagulans
55
-
-
Bacillus sp. (in: Bacteria)
55
-
-
Niallia circulans
55
-
-
Paenibacillus macerans
55
-
-
Bacillus ohbensis
60
-
-
Geobacillus stearothermophilus
60
-
-
Bacillus sp. (in: Bacteria)
60
-
-
Niallia circulans
60
-
-
Paenibacillus macerans
60
-
-
Bacillus ohbensis
65
-
-
Bacillus subtilis
65
-
-
Geobacillus stearothermophilus
70
-
-
Geobacillus stearothermophilus
70
-
-
Weizmannia coagulans
70
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
Weizmannia coagulans
30 70
-
Niallia circulans
30 70
-
Paenibacillus macerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 does not lose activity by heat treatment for 10 min at 70-75°C, pH 5.5-9.5 in presence of CaCl2, loses half of the activity by heating at 80°C for 10 min Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 4.7
-
Niallia circulans
5 5.7
-
Priestia megaterium
5 5.7
-
Bacillus sp. (in: Bacteria)
5 5.7
-
Paenibacillus macerans
5.5
-
-
Geobacillus stearothermophilus
5.5
-
-
Niallia circulans
5.5
-
-
Paenibacillus macerans
5.5
-
-
Bacillus ohbensis
6
-
-
Geobacillus stearothermophilus
6
-
-
Weizmannia coagulans
6
-
-
Bacillus sp. (in: Bacteria)
6
-
-
Niallia circulans
7
-
-
Priestia megaterium
7
-
-
Niallia circulans
7
-
-
Paenibacillus macerans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 activity is not lost by incubation at 4°C for 1 month Weizmannia coagulans
5.5 9.5 stable at 70°C Weizmannia coagulans
6 9 stable at 70°C Niallia circulans
6 8
-
Niallia circulans
6 8 stable at 50°C Bacillus subtilis
6 10
-
Weizmannia coagulans
6 10 stable at 65°C Niallia circulans
6 9.5 stable at 55°C Niallia circulans
6.5 8.5 stable at 50°C Paenibacillus macerans
6.5 9.5 stable at 55°C Bacillus ohbensis
7 10
-
Bacillus sp. (in: Bacteria)
7 10 stable at 65°C Priestia megaterium
7 9.2 stable at 50°C Geobacillus stearothermophilus