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Literature summary for 2.4.1.188 extracted from
- Solubilization and properties of UDP-D-glucose:N-acetylglucosaminyl pyrophosphorylundecaprenol glucosyltransferase from Bacillus coagulans AHU 1366 membranes (1988), J. Biochem., 104, 985-988.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | stimulation | Weizmannia coagulans |  |
| glycerol | at 20% stimulates 3fold | Weizmannia coagulans | |
| KCl | at 0.6 M stimulation | Weizmannia coagulans | |
| MgCl2 | at 40 mM stimulation | Weizmannia coagulans | |
| NaCl | stimulation | Weizmannia coagulans | |
| NH4Cl | stimulation | Weizmannia coagulans | |
| Nonidet P-40 | at 0.1% slight stimulatory | Weizmannia coagulans | |
General Stability
| General Stability | Organism |
|---|---|
| stabilization of the solubilized enzyme by cations and glycerol | Weizmannia coagulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
UDP-glucose | - |
Weizmannia coagulans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Weizmannia coagulans | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol | Weizmannia coagulans | - |
UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol | - |
? | |
| UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol | Weizmannia coagulans AHU 1366 | - |
UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Weizmannia coagulans | - |
AHU 1366 | - |
| Weizmannia coagulans AHU 1366 | - |
AHU 1366 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Weizmannia coagulans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, several months without significant loss of activity | Weizmannia coagulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol | - |
Weizmannia coagulans | UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol | - |
? | |
| UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol | - |
Weizmannia coagulans AHU 1366 | UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Weizmannia coagulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 8 | - |
Weizmannia coagulans |
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