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Literature summary for 2.4.1.185 extracted from
- Flavanone-7-O-glucosyltransferase activity from Petunia hybrida (1999), Phytochemistry, 52, 793-798.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | competitive inhibition | Petunia x hybrida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
naringenin | peak III | Petunia x hybrida | |
| 0.01 | - |
naringenin | peak I | Petunia x hybrida | |
| 0.027 | - |
naringenin | peak II | Petunia x hybrida | |
| 0.074 | - |
UDPglucose | peak II | Petunia x hybrida | |
| 0.269 | - |
UDPglucose | peak I | Petunia x hybrida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is not affected by CaCl2 or MgCl2 | Petunia x hybrida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54400 | - |
gel filtration | Petunia x hybrida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone | Petunia x hybrida | peak I-III | UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petunia x hybrida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Superose 12, Mono Q, 3 peaks with 7-O-glucosyltransferase activity | Petunia x hybrida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | - |
Petunia x hybrida | - |
| leaf | - |
Petunia x hybrida | - |
| sepal | - |
Petunia x hybrida | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone | peak I-III | Petunia x hybrida | UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + apigenin | peak I-III, 49%, 45% and 95% of activity with naringenin respectively | Petunia x hybrida | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + kaempferol | peak I-III, 50%, 46% and 70% of activity with naringenin respectively | Petunia x hybrida | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + luteolin | peak I, 52% of activity with naringenin | Petunia x hybrida | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + quercetin | peak I, 42% of activity with naringenin | Petunia x hybrida | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + hesperetin | peak I, 70% of activity with naringenin | Petunia x hybrida | UDP + hesperetin 7-O-glucoside | - |
? | |
| UDP-glucose + naringenin | - |
Petunia x hybrida | UDP + naringenin 7-O-glucoside | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
50% of maximal activity at pH 6.5, 62% of maximal activity at pH 9.0 | Petunia x hybrida |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00089 | - |
UDP | peak I | Petunia x hybrida | |
| 0.074 | - |
UDP | peak II | Petunia x hybrida | |
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