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Literature summary for 2.4.1.185 extracted from

  • McIntosh, C.A.; Mansell R.L.
    Biosynthesis of naringin in Citrus paradisi: UDP-glucosyl-transferase activity in grapefruit seedlings (1990), Phytochemistry, 29, 1533-1538.
No PubMed abstract available
Search for more literature for 2.4.1.185

Inhibitors

Inhibitors Comment Organism Structure
ADP 10 mM, strong Citrus x paradisi
ATP 10 mM, strong Citrus x paradisi
NADPH 10 mM, weak Citrus x paradisi
TTP 10 mM, strong Citrus x paradisi
UDP 10 mM, weak Citrus x paradisi
UTP 10 mM, strong Citrus x paradisi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 naringenin
-
 Citrus x paradisi
0.11
-
 hesperetin
-
 Citrus x paradisi
0.13
-
 2',4',6',4-tetrahydroxy chalcone
-
 Citrus x paradisi
0.37
-
 UDPglucose
-
 Citrus x paradisi

Metals/Ions

Metals/Ions Comment Organism Structure
additional information CaCl2, MnCl2, NaCl, MgCl2 at 0.1 mM, 1.0 mM and 10 mM have no effect Citrus x paradisi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone Citrus x paradisi trivial name naringenin UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Citrus x paradisi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrus x paradisi

Source Tissue

Source Tissue Comment Organism Textmining
seedling highest activity in leaves, more than in stem and roots Citrus x paradisi
-

Storage Stability

Storage Stability Organism
4°C, 1% bovine serum albumin, 2-3 days, no loss of activity Citrus x paradisi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone trivial name naringenin Citrus x paradisi UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + kaempferol 90% of activity with naringenin Citrus x paradisi UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + quercetin 164% of activity with naringenin Citrus x paradisi UDP + quercetin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + hesperetin
-
 Citrus x paradisi UDP + hesperetin 7-O-glucoside
-
 ?
UDP-glucose + naringenin
-
 Citrus x paradisi UDP + naringenin 7-O-glucoside
-
 ?
UDPglucose + 2',4',6',4-tetrahydroxychalcone 64% of activity with naringenin Citrus x paradisi UDP + 2',4',6',4-tetrahydroxychalcone 4'-O-glucoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Citrus x paradisi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Citrus x paradisi